Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device


Thomas, Rupert S.W., Mitchell, Peter D., Oreffo, Richard O. and Morgan, Hywel (2010) Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device [in special issue: Dielectrophoresis] Biomicrofluidics, 4, (2), p. 22806. (doi:10.1063/1.3406951). (PMID:20697594).

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Description/Abstract

We describe a system for the isolation, concentration, separation, and recovery of human osteoblast-like cells from a heterogeneous population using dielectrophoretic ring traps. Cells flowing in a microfluidic channel are immobilized inside an electric field cage using negative dielectrophoresis. A planar ring electrode creates a closed trap while repelling surrounding cells. Target cells are identified by fluorescent labeling, and are trapped as they pass across a ring electrode by an automated system. We demonstrate recovery of small populations of human osteoblast-like cells with a purity of 100%, which in turn demonstrates the potential of such a device for cell selection from a heterogeneous population.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1063/1.3406951
ISSNs: 1932-1058 (print)
Subjects:

ePrint ID: 181661
Date :
Date Event
June 2010Submitted
Date Deposited: 18 Apr 2011 14:35
Last Modified: 18 Apr 2017 02:27
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/181661

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