Thomas, Rupert S.W., Mitchell, Peter D., Oreffo, Richard O. and Morgan, Hywel
Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device
[in special issue: Dielectrophoresis]
Biomicrofluidics, 4, (2), . (doi:10.1063/1.3406951). (PMID:20697594).
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We describe a system for the isolation, concentration, separation, and recovery of human osteoblast-like cells from a heterogeneous population using dielectrophoretic ring traps. Cells flowing in a microfluidic channel are immobilized inside an electric field cage using negative dielectrophoresis. A planar ring electrode creates a closed trap while repelling surrounding cells. Target cells are identified by fluorescent labeling, and are trapped as they pass across a ring electrode by an automated system. We demonstrate recovery of small populations of human osteoblast-like cells with a purity of 100%, which in turn demonstrates the potential of such a device for cell selection from a heterogeneous population.
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