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Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device

Thomas, Rupert S.W., Mitchell, Peter D., Oreffo, Richard O. and Morgan, Hywel (2010) Trapping single human osteoblast-like cells from a heterogeneous population using a dielectrophoretic microfluidic device [in special issue: Dielectrophoresis] Biomicrofluidics, 4, (2), p. 22806. (doi:10.1063/1.3406951). (PMID:20697594).

Record type: Article

Abstract

We describe a system for the isolation, concentration, separation, and recovery of human osteoblast-like cells from a heterogeneous population using dielectrophoretic ring traps. Cells flowing in a microfluidic channel are immobilized inside an electric field cage using negative dielectrophoresis. A planar ring electrode creates a closed trap while repelling surrounding cells. Target cells are identified by fluorescent labeling, and are trapped as they pass across a ring electrode by an automated system. We demonstrate recovery of small populations of human osteoblast-like cells with a purity of 100%, which in turn demonstrates the potential of such a device for cell selection from a heterogeneous population.

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Submitted date: June 2010

Identifiers

Local EPrints ID: 181661
URI: http://eprints.soton.ac.uk/id/eprint/181661
ISSN: 1932-1058
PURE UUID: e48c794c-2519-4c3e-a8b8-f45d509fc822
ORCID for Richard O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 18 Apr 2011 14:35
Last modified: 18 Jul 2017 11:58

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Contributors

Author: Rupert S.W. Thomas
Author: Peter D. Mitchell
Author: Hywel Morgan ORCID iD

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