Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells
Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells
Human skeletal stem cells (STRO-1 positive/STRO-1+) respond to different topographical features in various ways. On a flat surface these cells spread and tend to develop a fibroblast-like morphology. On a microgrooved surface enriched skeletal stem cell populations prefer to stretch along the grooves, which affects their cellular structure and differentiation, a phenomenon known as contact guidance. Growth factors, hormones and chemicals can also stimulate cell differentiation. A traditional Chinese medicine, puerariae radix, has previously been observed to stimulate bone formation. The active ingredients have been identified as isoflavones with estrogen-like bioactivity. This study combined the effects of microgrooved topology and hormone-like isoflavones in the biodegradable polymer polycaprolactone (PCL). Human osteogenic cells (STRO-1+) were cultured on flat PCL, grooved PCL and puerariae powder-impregnated grooved PCL for 5 weeks. Coomassie staining indicated that cell growth and survival was similar on flat PCL, grooved PCL and grooved PCL impregnated with 1 wt.% or 2 wt.% puerariae powder. Grooved PCL impregnated with 2 wt.% puerariae powder was observed to have an influence on protein expression, as observed by positive osteocalcin staining. Protein expression profiles were analyzed by difference gel electrophoresis to identify proteins that showed modulation of expression in response to these different environments. Overall, our results suggest that puerariae powder has an additive effect, along with microgrooved topographical stimulation, to promote changes in the STRO-1+ proteome that affect cell phenotype.
surface topography, human bone marrow stromal cells, puerariae radix, tissue engineering, differential proteomics
3694-3703
Kantawong, Fahsai
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Burgess, Karl E.V.
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Jayawardena, Kamburapola
0481e755-3e8d-42e0-947e-895de802c3f8
Hart, Andrew
6011e639-937a-4d81-aa83-ba1ad25ca916
Riehle, Mathias O.
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Oreffo, Richard O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Burchmore, Richard
967325b6-3596-4de0-be59-01ea5f3eaa71
September 2010
Kantawong, Fahsai
d209298e-ac00-49f0-a9b9-39d1e82d5925
Burgess, Karl E.V.
7eef15f0-15b2-4898-ac8a-bb5f49f8ff48
Jayawardena, Kamburapola
0481e755-3e8d-42e0-947e-895de802c3f8
Hart, Andrew
6011e639-937a-4d81-aa83-ba1ad25ca916
Riehle, Mathias O.
1236dddb-039c-44f6-8647-e7939b7f735d
Oreffo, Richard O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Burchmore, Richard
967325b6-3596-4de0-be59-01ea5f3eaa71
Kantawong, Fahsai, Burgess, Karl E.V., Jayawardena, Kamburapola, Hart, Andrew, Riehle, Mathias O., Oreffo, Richard O., Dalby, Matthew J. and Burchmore, Richard
(2010)
Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells.
Acta Biomaterialia, 6 (9), .
(doi:10.1016/j.actbio.2010.02.038).
(PMID:20302977)
Abstract
Human skeletal stem cells (STRO-1 positive/STRO-1+) respond to different topographical features in various ways. On a flat surface these cells spread and tend to develop a fibroblast-like morphology. On a microgrooved surface enriched skeletal stem cell populations prefer to stretch along the grooves, which affects their cellular structure and differentiation, a phenomenon known as contact guidance. Growth factors, hormones and chemicals can also stimulate cell differentiation. A traditional Chinese medicine, puerariae radix, has previously been observed to stimulate bone formation. The active ingredients have been identified as isoflavones with estrogen-like bioactivity. This study combined the effects of microgrooved topology and hormone-like isoflavones in the biodegradable polymer polycaprolactone (PCL). Human osteogenic cells (STRO-1+) were cultured on flat PCL, grooved PCL and puerariae powder-impregnated grooved PCL for 5 weeks. Coomassie staining indicated that cell growth and survival was similar on flat PCL, grooved PCL and grooved PCL impregnated with 1 wt.% or 2 wt.% puerariae powder. Grooved PCL impregnated with 2 wt.% puerariae powder was observed to have an influence on protein expression, as observed by positive osteocalcin staining. Protein expression profiles were analyzed by difference gel electrophoresis to identify proteins that showed modulation of expression in response to these different environments. Overall, our results suggest that puerariae powder has an additive effect, along with microgrooved topographical stimulation, to promote changes in the STRO-1+ proteome that affect cell phenotype.
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Published date: September 2010
Keywords:
surface topography, human bone marrow stromal cells, puerariae radix, tissue engineering, differential proteomics
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Local EPrints ID: 181667
URI: http://eprints.soton.ac.uk/id/eprint/181667
ISSN: 1742-7061
PURE UUID: 5fd5fb90-e979-4c27-b8fd-33a30c69bdcd
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Date deposited: 19 Apr 2011 10:54
Last modified: 15 Mar 2024 03:03
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Author:
Fahsai Kantawong
Author:
Karl E.V. Burgess
Author:
Kamburapola Jayawardena
Author:
Andrew Hart
Author:
Mathias O. Riehle
Author:
Matthew J. Dalby
Author:
Richard Burchmore
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