Jefferies, Johanna M.C., Tee, Nancy and Clarke, Stuart C.
Molecular analysis of Streptococcus pneumoniae clones causing invasive disease in children in Singapore
Journal of Medical Microbiology (doi:10.1099/jmm.0.030007-0). (PMID:21330410).
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Streptococcus pneumoniae remains a leading cause of serious paediatric disease. However there is little published epidemiological data regarding invasive pneumococcal disease (IPD) in many countries in South East Asia, including Singapore. Baseline data for IPD is essential to inform policy regarding pneumococcal conjugate vaccine use in Singapore. This is the first study to use multi-locus sequence typing (MLST) to investigate clonal relationships among Singaporean IPD isolates. We characterised 86 invasive pneumococci isolated from Singaporean children using serotyping and MLST. The objectives were to compare Singaporean MLST data to worldwide data and to assess serotype distribution in relation to current PCV formulations. We observed 49 sequence types (STs); a high proportion of which, (n=16) were novel STs. Despite the presence of these novel STs, serotype distribution was similar to that observed elsewhere. Serotypes 14, 6B, 19A and 19F accounted for 85% of IPD cases. PCV7, PCV10 and PCV13 covered 85%, 86% and 97% of IPD respectively. We have demonstrated a pressing need for larger studies to determine the molecular epidemiology and antibiotic susceptibility of circulating pneumococcal clones from both carriage and disease in Singapore.
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