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Evaluation of full-length, cleaved and nitrosylated serum surfactant protein D as biomarkers for COPD

Evaluation of full-length, cleaved and nitrosylated serum surfactant protein D as biomarkers for COPD
Evaluation of full-length, cleaved and nitrosylated serum surfactant protein D as biomarkers for COPD
Chronic obstructive pulmonary disease (COPD) is a multicomponent condition that is characterized by partially reversible airflow obstruction. Serum surfactant protein D (SP-D) is synthesized by type II pneumocytes and Clara cells and participates in surfactant homeostasis and pulmonary host defense. Serum levels of SP-D are raised in individuals with COPD but there is no correlation between the serum level of SP-D and the severity of airflow obstruction. Serum SP-D is present in different forms that may have more utility as a biomarker for COPD. We report here the development of new monoclonal antibodies to full length and cleaved SP-D. We have assessed these and existing antibodies in 98 individuals with COPD recruited to the Evaluation of COPD Longitudinally to Identify Predictive Surrogate Endpoints (ECLIPSE) cohort. Our data show that neither monoclonal antibodies to full length nor cleaved SP-D provide additional information over that obtained with a polyclonal antibody. Moreover, levels of serum nitrosylated-SP-D did not correlate with serum level of SP-D or any clinical phenotype of COPD. The measurement of modified SP-D is of limited value in characterising individuals with COPD.
surfactant protein d, copd, biomarker, monoclonal antibodies
1541-2555
79-95
Duvoix, Annelyse
f9113c62-9335-4c0b-b34b-294181995343
Miranda, Elena
3b02a7d4-d28a-45b8-bd7e-e2ab8dcad2d1
Perez, Juan
f1162e74-754e-4f15-8cea-7053610e2019
Sorensen, Grith L.
25ac3a15-3fc9-4dab-9fa5-caf89893b845
Holmskov, Uffe
8a55aecd-694c-4d61-849b-9cf32da8ba39
Trapnell, Bruce C.
58ac03f6-d757-4d39-a728-6e2fc46cec4a
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Clark, Howard W.
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Edwards, Lisa D.
8a222d5b-b9e8-425b-a4c9-21edd77e5ff2
Miller, Bruce E.
06a2a9a3-5f2b-4c77-9e9c-7947fc818596
Tal-Singer, Ruth M.
e3e87c1c-77c8-42fa-8536-d8394b2abaa0
Lomas, David A.
0641855a-442c-44b1-9684-f3e3cfc97808
Duvoix, Annelyse
f9113c62-9335-4c0b-b34b-294181995343
Miranda, Elena
3b02a7d4-d28a-45b8-bd7e-e2ab8dcad2d1
Perez, Juan
f1162e74-754e-4f15-8cea-7053610e2019
Sorensen, Grith L.
25ac3a15-3fc9-4dab-9fa5-caf89893b845
Holmskov, Uffe
8a55aecd-694c-4d61-849b-9cf32da8ba39
Trapnell, Bruce C.
58ac03f6-d757-4d39-a728-6e2fc46cec4a
Madsen, Jens
b5d8ae35-00ac-4d19-930e-d8ddec497359
Clark, Howard W.
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Edwards, Lisa D.
8a222d5b-b9e8-425b-a4c9-21edd77e5ff2
Miller, Bruce E.
06a2a9a3-5f2b-4c77-9e9c-7947fc818596
Tal-Singer, Ruth M.
e3e87c1c-77c8-42fa-8536-d8394b2abaa0
Lomas, David A.
0641855a-442c-44b1-9684-f3e3cfc97808

Duvoix, Annelyse, Miranda, Elena, Perez, Juan, Sorensen, Grith L., Holmskov, Uffe, Trapnell, Bruce C., Madsen, Jens, Clark, Howard W., Edwards, Lisa D., Miller, Bruce E., Tal-Singer, Ruth M. and Lomas, David A. (2011) Evaluation of full-length, cleaved and nitrosylated serum surfactant protein D as biomarkers for COPD. COPD: Journal of Chronic Obstructive Pulmonary Disease, 8 (2), 79-95. (doi:10.3109/15412555.2011.558542). (PMID:21495836)

Record type: Article

Abstract

Chronic obstructive pulmonary disease (COPD) is a multicomponent condition that is characterized by partially reversible airflow obstruction. Serum surfactant protein D (SP-D) is synthesized by type II pneumocytes and Clara cells and participates in surfactant homeostasis and pulmonary host defense. Serum levels of SP-D are raised in individuals with COPD but there is no correlation between the serum level of SP-D and the severity of airflow obstruction. Serum SP-D is present in different forms that may have more utility as a biomarker for COPD. We report here the development of new monoclonal antibodies to full length and cleaved SP-D. We have assessed these and existing antibodies in 98 individuals with COPD recruited to the Evaluation of COPD Longitudinally to Identify Predictive Surrogate Endpoints (ECLIPSE) cohort. Our data show that neither monoclonal antibodies to full length nor cleaved SP-D provide additional information over that obtained with a polyclonal antibody. Moreover, levels of serum nitrosylated-SP-D did not correlate with serum level of SP-D or any clinical phenotype of COPD. The measurement of modified SP-D is of limited value in characterising individuals with COPD.

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Published date: 1 April 2011
Keywords: surfactant protein d, copd, biomarker, monoclonal antibodies

Identifiers

Local EPrints ID: 182277
URI: http://eprints.soton.ac.uk/id/eprint/182277
ISSN: 1541-2555
PURE UUID: 47b41c8e-9e0f-4b5a-a43f-a7b068c54183
ORCID for Jens Madsen: ORCID iD orcid.org/0000-0003-1664-7645

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Date deposited: 27 Apr 2011 14:06
Last modified: 15 Mar 2024 03:29

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Contributors

Author: Annelyse Duvoix
Author: Elena Miranda
Author: Juan Perez
Author: Grith L. Sorensen
Author: Uffe Holmskov
Author: Bruce C. Trapnell
Author: Jens Madsen ORCID iD
Author: Howard W. Clark
Author: Lisa D. Edwards
Author: Bruce E. Miller
Author: Ruth M. Tal-Singer
Author: David A. Lomas

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