The University of Southampton
University of Southampton Institutional Repository

The phytochrome-deficient pcd2 mutant of pea is unable to convert biliverdin IXalpha to 3(Z)-phytochromobilin

Weller, James L., Terry, Matthew J., Reid, James B. and Kendrick, Richard E. (1997) The phytochrome-deficient pcd2 mutant of pea is unable to convert biliverdin IXalpha to 3(Z)-phytochromobilin The Plant Journal, 11, (6), pp. 1177-1186. (doi:10.1046/j.1365-313X.1997.11061177.x).

Record type: Article

Abstract

During screening of ethylmethane sulphonate-mutagenized pea (Pisum sativum L.) seedlings under far-red light a mutant line, AF130, was isolated which showed a reduction in both red and far-red light-induced de-etiolation responses. The photomorphogenic phenotype of AF130 results from a single recessive mutation which is not allelic with the previously described phytochrome chromophore biosynthesis mutant pcd1. This new mutant has been designated pcd2, for phytochrome chromophore deficient 2. Like pcd1, etiolated pcd2 seedlings are severely deficient in spectrally active phytochrome and contain wild-type levels of phytochrome A apoprotein which is not substantially depleted by red light treatment. Etioplast preparations from pcd2 seedlings can metabolize heme to biliverdin (BV) IX?, but are unable to convert BV IX? to the phytochrome chromophore, phytochromobilin. The PCD1 and PCD2 genes therefore control consecutive steps in phytochromobilin synthesis. Despite a similarly severe impairment of photomorphogenic responses, pcd2 mutant seedlings do not display the strongly chlorotic phenotype of pcd1, suggesting that this characteristic of pcd1 does not result from phytochrome deficiency per se, but is a specific effect of the pcd1 mutation. A double mutant between pcd1 and pcd2 was constructed. This mutant is paler than pcd1 and less responsive to red light than either single mutant, but retains a strong response to blue light.

Full text not available from this repository.

More information

Published date: June 1997

Identifiers

Local EPrints ID: 182695
URI: http://eprints.soton.ac.uk/id/eprint/182695
ISSN: 0960-7412
PURE UUID: 4ddaf565-1b69-47da-a2b3-b1d21feb7916

Catalogue record

Date deposited: 18 May 2011 09:19
Last modified: 18 Jul 2017 11:56

Export record

Altmetrics

Contributors

Author: James L. Weller
Author: James B. Reid
Author: Richard E. Kendrick

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×