Basophil recruitment and activation in inflammatory skin diseases
Basophil recruitment and activation in inflammatory skin diseases
Background:? Basophils are blood leukocytes constituting less than 1% of leukocytes. They share morphological and functional similarities with mast cells, but recent studies indicate that basophils play non-redundant roles via the release of several cytokines and lipid mediators, as well as functioning as antigen presenting cells. However, basophil infiltration into the tissues in human skin diseases remains to be addressed.
Methods:? The infiltration of basophils in 24 skin diseases (136 samples) was immunohistochemically analyzed using basophil-specific BB1 antibody. In addition, activation of blood basophils was examined by assessing CD203c expression with flow cytometry.
Results:? Basophils were detected in skin lesions of atopic dermatitis, prurigo, urticaria, bullous pemphigoid, drug eruptions, eosinophilic pustular folliculitis, insect bites, scabies, Henoch–Schönlein purpura and dermatomyositis. While cell densities in urticaria, bullous pemphigoid and eosinophilic pustular folliculitis were prominent, much lower numbers of basophils were seen in lesional skin of atopic dermatitis. Basophils were entirely absent in psoriasis vulgaris, mastocytosis, tumoral lesions, systemic sclerosis, and systemic lupus erythematosus. Levels of CD203c expression on blood basophils from prurigo and urticaria patients were higher than those from healthy donors.
Conclusions:? Basophils infiltrate into skin lesions more commonly than previously thought, and thus they may play important roles in a variety of inflammatory skin diseases.
atopic dermatitis, bb1, cd203c, prurigo, urticaria
1107-1113
Ito, Y.
7637b3e6-96a0-43e4-9702-eb3f5b55c86c
Satoh, T.
0d9ce2c9-b003-498d-b95c-607b69bf0cd3
Takayama, K.
5ce4b71e-6076-48a0-b142-b89ce2a23621
Miyagishi, C.
da9040af-2921-459a-b95c-1edbc2dfb46e
Walls, A.F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Yokozeki, H.
3bdce23a-c988-4ccc-b211-faf484d6cc80
August 2011
Ito, Y.
7637b3e6-96a0-43e4-9702-eb3f5b55c86c
Satoh, T.
0d9ce2c9-b003-498d-b95c-607b69bf0cd3
Takayama, K.
5ce4b71e-6076-48a0-b142-b89ce2a23621
Miyagishi, C.
da9040af-2921-459a-b95c-1edbc2dfb46e
Walls, A.F.
aaa7e455-0562-4b4c-94f5-ec29c74b1bfe
Yokozeki, H.
3bdce23a-c988-4ccc-b211-faf484d6cc80
Ito, Y., Satoh, T., Takayama, K., Miyagishi, C., Walls, A.F. and Yokozeki, H.
(2011)
Basophil recruitment and activation in inflammatory skin diseases.
Allergy, 66 (8), .
(doi:10.1111/j.1398-9995.2011.02570.x).
(PMID:21371044)
Abstract
Background:? Basophils are blood leukocytes constituting less than 1% of leukocytes. They share morphological and functional similarities with mast cells, but recent studies indicate that basophils play non-redundant roles via the release of several cytokines and lipid mediators, as well as functioning as antigen presenting cells. However, basophil infiltration into the tissues in human skin diseases remains to be addressed.
Methods:? The infiltration of basophils in 24 skin diseases (136 samples) was immunohistochemically analyzed using basophil-specific BB1 antibody. In addition, activation of blood basophils was examined by assessing CD203c expression with flow cytometry.
Results:? Basophils were detected in skin lesions of atopic dermatitis, prurigo, urticaria, bullous pemphigoid, drug eruptions, eosinophilic pustular folliculitis, insect bites, scabies, Henoch–Schönlein purpura and dermatomyositis. While cell densities in urticaria, bullous pemphigoid and eosinophilic pustular folliculitis were prominent, much lower numbers of basophils were seen in lesional skin of atopic dermatitis. Basophils were entirely absent in psoriasis vulgaris, mastocytosis, tumoral lesions, systemic sclerosis, and systemic lupus erythematosus. Levels of CD203c expression on blood basophils from prurigo and urticaria patients were higher than those from healthy donors.
Conclusions:? Basophils infiltrate into skin lesions more commonly than previously thought, and thus they may play important roles in a variety of inflammatory skin diseases.
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e-pub ahead of print date: 4 March 2011
Published date: August 2011
Keywords:
atopic dermatitis, bb1, cd203c, prurigo, urticaria
Organisations:
Infection Inflammation & Immunity, Clinical & Experimental Sciences
Identifiers
Local EPrints ID: 183787
URI: http://eprints.soton.ac.uk/id/eprint/183787
ISSN: 0105-4538
PURE UUID: 8638a716-0be7-4212-8227-552517d8648e
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Date deposited: 04 May 2011 09:25
Last modified: 15 Mar 2024 02:39
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Contributors
Author:
Y. Ito
Author:
T. Satoh
Author:
K. Takayama
Author:
C. Miyagishi
Author:
H. Yokozeki
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