The University of Southampton
University of Southampton Institutional Repository

Biphasic production of hydrogen and methane from waste lactose in cyclic-batch reactors

Banks, Charles J., Zotova, Elina A and Heaven, Sonia (2010) Biphasic production of hydrogen and methane from waste lactose in cyclic-batch reactors [in special issue: Sustainable Hydrogen from Biomass] Journal of Cleaner Production, 18, supplement 1, S95-S104. (doi:10.1016/j.jclepro.2010.04.018).

Record type: Article


The biphasic production of the energy gases hydrogen and methane was possible in a fed batch culture resulting in a volumetric mix of approximately 20% H2 and 80% CH4 and an energy conversion efficiency of 95%, based on the measured Chemical Oxygen Demand and theoretical calculations assuming that the substrate (a dairy waste permeate) was lactose. Gas production showed a rapid initial phase over 0–20 h in which the composition was up to 50% hydrogen with the balance mainly carbon dioxide. This was accompanied by the accumulation of volatile fatty acids (VFA) in which butyric was predominant. A slower second phase of gas production produced a mixture of methane and carbon dioxide with a reduction in the accumulated acids. The duration of this second phase depended on the initial load applied to the reactor, and in the experiments carried out lasted between 6 and 12 days. Where the applied initial load led to an acid accumulation such that the pH fell below 5.5, the second phase of gas production was inhibited. Where pH control was exerted to prevent the pH dropping below 6.5, ethanol accumulated alongside VFA as a first phase product, with the gas comprised entirely of carbon dioxide. Despite the excellent energy conversion and the production of biogas fuel elements matching those for hythane (a mixture of hydrogen and methane, with improved combustion characteristics), the overall process loading was considered too low for efficient volumetric conversion of the feedstock to energy. The concept could be further developed based on high rate reactor systems with granular or immobilised biomass either as a single tank biphasic system or in a split tank two phase production process.

Full text not available from this repository.

More information

Published date: December 2010
Keywords: hydrogen production, hythane, anaerobic digestion, dairy waste, lactose


Local EPrints ID: 184687
ISSN: 0959-6526
PURE UUID: fb247333-2276-4f19-a883-58691c9ad70e

Catalogue record

Date deposited: 06 May 2011 10:34
Last modified: 18 Jul 2017 11:51

Export record


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.