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Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network trafficking and cell elongation

Gendre, Delphine, Oh, Jaesung, Boutte, Yuhann, Best, Jacob G., Samuels, Lacey, Nilsson, Robert, Uemura, Tomohiro, Marchant, Alan, Bennett, Malcolm J., Grebe, Markus and Bhalerao, Rishikesh P. (2011) Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network trafficking and cell elongation Proceedings of the National Academy of Sciences, 108, (19), pp. 8048-8053. (doi:10.1073/pnas.1018371108). (PMID:21512130).

Record type: Article

Abstract

Multiple steps of plant growth and development rely on rapid cell elongation during which secretory and endocytic trafficking via the trans-Golgi network (TGN) plays a central role. Here, we identify the ECHIDNA (ECH) protein from Arabidopsis thaliana as a TGN-localized component crucial for TGN function. ECH partially complements loss of budding yeast TVP23 function and a Populus ECH complements the Arabidopsis ech mutant, suggesting functional conservation of the genes. Compared with wild-type, the Arabidopsis ech mutant exhibits severely perturbed cell elongation as well as defects in TGN structure and function, manifested by the reduced association between Golgi bodies and TGN as well as mislocalization of several TGN-localized proteins including vacuolar H+-ATPase subunit a1 (VHA-a1). Strikingly, ech is defective in secretory trafficking, whereas endocytosis appears unaffected in the mutant. Some aspects of the ech mutant phenotype can be phenocopied by treatment with a specific inhibitor of vacuolar H+-ATPases, concanamycin A, indicating that mislocalization of VHA-a1 may account for part of the defects in ech. Hence, ECH is an evolutionarily conserved component of the TGN with a central role in TGN structure and function.

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More information

Published date: 21 April 2011
Keywords: secretory pathway, dwarf mutant, vacuolar defect
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 184787
URI: http://eprints.soton.ac.uk/id/eprint/184787
ISSN: 0027-8424
PURE UUID: 420b209b-e345-4b7a-bd94-16f2d14e0e89

Catalogue record

Date deposited: 09 May 2011 09:27
Last modified: 18 Jul 2017 11:50

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Contributors

Author: Delphine Gendre
Author: Jaesung Oh
Author: Yuhann Boutte
Author: Jacob G. Best
Author: Lacey Samuels
Author: Robert Nilsson
Author: Tomohiro Uemura
Author: Alan Marchant
Author: Malcolm J. Bennett
Author: Markus Grebe
Author: Rishikesh P. Bhalerao

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