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Numerical analysis of ischemia- and compression-induced injury in tissue-engineered skeletal muscle constructs

Numerical analysis of ischemia- and compression-induced injury in tissue-engineered skeletal muscle constructs
Numerical analysis of ischemia- and compression-induced injury in tissue-engineered skeletal muscle constructs
Pressure-related deep tissue injury may develop in skeletal muscle tissue which is subjected to prolonged compression. For early detection, it is important to understand the underlying damage processes. Gawlitta et al. [Gawlitta, D., C. W. J. Oomens, D. L. Bader, F. P. T. Baaijens, and C. V. C. Bouten. Temporal differences in the influence of ischemic factors and deformation on the metabolism of engineered skeletal muscle. J. Appl. Physiol. 103(2):464–473, 2007b] subjected tissue-engineered muscle constructs to ischemia and deformation to study their effects on viability. Contrary to previous findings, no decrease in viability was found due to compression. However, the nature of their measurement method complicated interpretation of the results, particulary when deformation was involved. Changes in the constructs were assessed by measurements in the surrounding medium. The theoretical model developed in the present study describes metabolism, diffusion, and cell death in the experiments, and accounts for reduced diffusion due to compression. It was demonstrated that the lack of effect of compression on tissue viability, as measured in the experiments, could be explained by the compression-induced decrease in diffusivity. Compression did lead to considerable cell death but this could not be measured by Gawlitta et al. [Gawlitta, D., C. W. J. Oomens, D. L. Bader, F. P. T. Baaijens, and C. V. C. Bouten. Temporal differences in the influence of ischemic factors and deformation on the metabolism of engineered skeletal muscle. J. Appl. Physiol. 103(2):464–473, 2007b] because diffusion of the cell death marker to the medium was limited. This study shows that a proper description of transport processes is essential for a correct interpretation of experiments in which indirect measurement methods are used.
0090-6964
570-582
Ceelen, Karlien K.
50f2ec97-d334-4a68-88b2-5c2a4392f2e9
Gawlitta, D.
80fbcc4a-2719-4716-a94c-c084b91bb6fa
Bader, D.L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Oomens, C.W.J.
a8310c52-8ab4-4652-b2d6-82269a3c7438
Ceelen, Karlien K.
50f2ec97-d334-4a68-88b2-5c2a4392f2e9
Gawlitta, D.
80fbcc4a-2719-4716-a94c-c084b91bb6fa
Bader, D.L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Oomens, C.W.J.
a8310c52-8ab4-4652-b2d6-82269a3c7438

Ceelen, Karlien K., Gawlitta, D., Bader, D.L. and Oomens, C.W.J. (2010) Numerical analysis of ischemia- and compression-induced injury in tissue-engineered skeletal muscle constructs. Annals of Biomedical Engineering, 38 (3), 570-582. (doi:10.1007/s10439-009-9859-y).

Record type: Article

Abstract

Pressure-related deep tissue injury may develop in skeletal muscle tissue which is subjected to prolonged compression. For early detection, it is important to understand the underlying damage processes. Gawlitta et al. [Gawlitta, D., C. W. J. Oomens, D. L. Bader, F. P. T. Baaijens, and C. V. C. Bouten. Temporal differences in the influence of ischemic factors and deformation on the metabolism of engineered skeletal muscle. J. Appl. Physiol. 103(2):464–473, 2007b] subjected tissue-engineered muscle constructs to ischemia and deformation to study their effects on viability. Contrary to previous findings, no decrease in viability was found due to compression. However, the nature of their measurement method complicated interpretation of the results, particulary when deformation was involved. Changes in the constructs were assessed by measurements in the surrounding medium. The theoretical model developed in the present study describes metabolism, diffusion, and cell death in the experiments, and accounts for reduced diffusion due to compression. It was demonstrated that the lack of effect of compression on tissue viability, as measured in the experiments, could be explained by the compression-induced decrease in diffusivity. Compression did lead to considerable cell death but this could not be measured by Gawlitta et al. [Gawlitta, D., C. W. J. Oomens, D. L. Bader, F. P. T. Baaijens, and C. V. C. Bouten. Temporal differences in the influence of ischemic factors and deformation on the metabolism of engineered skeletal muscle. J. Appl. Physiol. 103(2):464–473, 2007b] because diffusion of the cell death marker to the medium was limited. This study shows that a proper description of transport processes is essential for a correct interpretation of experiments in which indirect measurement methods are used.

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Published date: March 2010

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Local EPrints ID: 185097
URI: https://eprints.soton.ac.uk/id/eprint/185097
ISSN: 0090-6964
PURE UUID: ba5583d5-30fc-4299-a786-16fe689ac36f
ORCID for D.L. Bader: ORCID iD orcid.org/0000-0002-1208-3507

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Date deposited: 09 May 2011 12:58
Last modified: 15 Aug 2019 00:38

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Contributors

Author: Karlien K. Ceelen
Author: D. Gawlitta
Author: D.L. Bader ORCID iD
Author: C.W.J. Oomens

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