Elevation of sputum matrix metalloproteinase-9 persists up to 6 months after smoking cessation: a research study
Elevation of sputum matrix metalloproteinase-9 persists up to 6 months after smoking cessation: a research study
Background: Smoking cessation is the best possible way to prevent the progression of smoking related airway diseases. However, the effect and time scale of smoking cessation on airway inflammation/remodelling are largely unknown. This prospective study evaluated the effects of smoking cessation on induced sputum (IS) neutrophils, matrix metalloproteinases (MMP-7, -8, -9) and tissue inhibitor of metalloproteinase-1 (TIMP-1).
Methods: A total of 61 subjects participated in the study; 17 stopped smoking for 3 months and 9 for 6 months. The proportion of IS neutrophils and the levels of MMPs and TIMP-1 by ELISA were determined at baseline and at 3 and 6 months after cessation.
Results: In the smokers, baseline IS neutrophils, MMPs and TIMP-1 were significantly higher compared to non-smokers. Levels of MMP-7, -8 and TIMP-1 decreased nearly to those of non-smokers but the levels of MMP-9 increased significantly from the baseline of the same subjects at 3 months after cessation (p = 0.009) with no significant decline at 6 months after cessation.
Conclusions: Sputum MMP-9 remained elevated after 6 months of smoking cessation, which may contribute to ongoing lung damage typical of COPD.
13
Louhelainen, Noora
a4f2a7ee-bbba-4e6a-8e07-dfecf6a0552c
Stark, Harri
ed07d994-5363-42fc-afb7-e3496095d319
Mazur, Witold
7db1427f-aeea-4dcc-a98b-4018cda038bf
Rytilä, Paula
31115afe-9e77-46f6-b737-378eb5e8d474
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Kinnula, Vuokko L.
d9e2667b-498e-44bd-9500-f76b811a29fe
14 March 2010
Louhelainen, Noora
a4f2a7ee-bbba-4e6a-8e07-dfecf6a0552c
Stark, Harri
ed07d994-5363-42fc-afb7-e3496095d319
Mazur, Witold
7db1427f-aeea-4dcc-a98b-4018cda038bf
Rytilä, Paula
31115afe-9e77-46f6-b737-378eb5e8d474
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Kinnula, Vuokko L.
d9e2667b-498e-44bd-9500-f76b811a29fe
Louhelainen, Noora, Stark, Harri, Mazur, Witold, Rytilä, Paula, Djukanovic, Ratko and Kinnula, Vuokko L.
(2010)
Elevation of sputum matrix metalloproteinase-9 persists up to 6 months after smoking cessation: a research study.
BMC Pulmonary Medicine, 10, .
(doi:10.1186/1471-2466-10-13).
(PMID:7767520)
Abstract
Background: Smoking cessation is the best possible way to prevent the progression of smoking related airway diseases. However, the effect and time scale of smoking cessation on airway inflammation/remodelling are largely unknown. This prospective study evaluated the effects of smoking cessation on induced sputum (IS) neutrophils, matrix metalloproteinases (MMP-7, -8, -9) and tissue inhibitor of metalloproteinase-1 (TIMP-1).
Methods: A total of 61 subjects participated in the study; 17 stopped smoking for 3 months and 9 for 6 months. The proportion of IS neutrophils and the levels of MMPs and TIMP-1 by ELISA were determined at baseline and at 3 and 6 months after cessation.
Results: In the smokers, baseline IS neutrophils, MMPs and TIMP-1 were significantly higher compared to non-smokers. Levels of MMP-7, -8 and TIMP-1 decreased nearly to those of non-smokers but the levels of MMP-9 increased significantly from the baseline of the same subjects at 3 months after cessation (p = 0.009) with no significant decline at 6 months after cessation.
Conclusions: Sputum MMP-9 remained elevated after 6 months of smoking cessation, which may contribute to ongoing lung damage typical of COPD.
This record has no associated files available for download.
More information
Published date: 14 March 2010
Identifiers
Local EPrints ID: 185155
URI: http://eprints.soton.ac.uk/id/eprint/185155
ISSN: 1471-2466
PURE UUID: 95988168-13ac-4049-a748-78c78fe05180
Catalogue record
Date deposited: 09 May 2011 15:06
Last modified: 15 Mar 2024 02:36
Export record
Altmetrics
Contributors
Author:
Noora Louhelainen
Author:
Harri Stark
Author:
Witold Mazur
Author:
Paula Rytilä
Author:
Vuokko L. Kinnula
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics