The University of Southampton
University of Southampton Institutional Repository

A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development

Macklon, N.S., Pieters, M.H.E.C., Hassan, M.A., Jeucken, P.H.M., Eijkemans, M.J.C. and Fauser, B.C.J.M. (2002) A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development Human Reproduction, 17, (10), pp. 2700-2705. (doi:10.1093/humrep/17.10.2700). (PMID:12351551).

Record type: Article

Abstract

Background: Extending the period of in-vitro culture to the blastocyst stage may improve implantation rates in IVF treatment. Recognition of the dynamic nature of early embryo metabolism has led to the development of commercially available sequential culture systems. However, their improved efficacy over monoculture systems remains to be demonstrated in prospective studies.

Methods: Embryos obtained from 158 women undergoing IVF treatment were randomized by sealed envelopes to culture in one of three systems: (A) culture for 5 days in our own monoculture medium (Rotterdam medium); (B) culture for 3 days in Rotterdam medium followed by 2 days in fresh Rotterdam medium; (C) culture for 5 days using the commercially available G1/G2 sequential culture system.

Results: There were no significant differences in blastulation, implantation or pregnancy rates between the three tested culture systems.

Conclusion: The employed monoculture system is as effective as the G1/G2 sequential system for the culture of blastocysts for IVF.

Full text not available from this repository.

More information

Published date: October 2002
Keywords: blastocyst, culture medium, ivf, pregnancy rates, randomized controlled trial

Identifiers

Local EPrints ID: 185661
URI: http://eprints.soton.ac.uk/id/eprint/185661
PURE UUID: d7555b55-ecee-406f-8dfd-711b27a87f90

Catalogue record

Date deposited: 20 May 2011 09:05
Last modified: 18 Jul 2017 11:48

Export record

Altmetrics

Contributors

Author: N.S. Macklon
Author: M.H.E.C. Pieters
Author: M.A. Hassan
Author: P.H.M. Jeucken
Author: M.J.C. Eijkemans
Author: B.C.J.M. Fauser

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×