The University of Southampton
University of Southampton Institutional Repository

Role of STAT3 in glucocorticoid-induced expression of the human IL-10 gene

Record type: Article

In the present report we have determined the molecular mechanisms, which govern the expression of the human IL-10 gene when induced by the glucocorticoid Methyl-Prednisolone (MP). Treatment of cells with MP at 10(-6) M will readily induce IL-10 in CD19+ primary B cells and in a human B cell line. Analysis of the IL-10 promoter showed a robust 18-fold induction and demonstrated that a potential GRE motif was not required, while mutation of the -120 STAT-motif strongly reduced MP-induced trans-activation. A strong induction was also seen with a trimeric STAT-motif and over-expression of dominant-negative STAT3 could block MP induction of IL-10 mRNA. Finally, MP treatment induced binding of STAT3 to the promoter as shown by gelshift, supershift and by chromatin-immunoprecipitation. These data show that glucocorticoid-induced expression of the IL-10 gene is mediated by the transcription factor STAT3.

Full text not available from this repository.

Citation

Unterberger, Claudia, Staples, Karl J., Smallie, Timothy, Williams, Lynn, Foxwell, Brian, Schaefer, Annette, Kempkes, Bettina, Hofer, T.P.J., Koeppel, Max, Lohrum, Marion, Stunnenberg, Henk, Frankenberger, Marion and Ziegler-Heitbrock, Loems (2008) Role of STAT3 in glucocorticoid-induced expression of the human IL-10 gene Molecular Immunology, 45, (11), pp. 3230-3237. (doi:10.1016/j.molimm.2008.02.020). (PMID:18403020).

More information

Published date: June 2008

Identifiers

Local EPrints ID: 185833
URI: http://eprints.soton.ac.uk/id/eprint/185833
ISSN: 0161-5890
PURE UUID: 5a6a3004-33dd-4e69-b063-e7288a5b775d
ORCID for Karl J. Staples: ORCID iD orcid.org/0000-0003-3844-6457

Catalogue record

Date deposited: 11 May 2011 10:16
Last modified: 18 Apr 2017 02:15

Export record

Altmetrics

Contributors

Author: Claudia Unterberger
Author: Karl J. Staples ORCID iD
Author: Timothy Smallie
Author: Lynn Williams
Author: Brian Foxwell
Author: Annette Schaefer
Author: Bettina Kempkes
Author: T.P.J. Hofer
Author: Max Koeppel
Author: Marion Lohrum
Author: Henk Stunnenberg
Author: Marion Frankenberger
Author: Loems Ziegler-Heitbrock

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×