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Role of STAT3 in glucocorticoid-induced expression of the human IL-10 gene

Unterberger, Claudia, Staples, Karl J., Smallie, Timothy, Williams, Lynn, Foxwell, Brian, Schaefer, Annette, Kempkes, Bettina, Hofer, T.P.J., Koeppel, Max, Lohrum, Marion, Stunnenberg, Henk, Frankenberger, Marion and Ziegler-Heitbrock, Loems (2008) Role of STAT3 in glucocorticoid-induced expression of the human IL-10 gene Molecular Immunology, 45, (11), pp. 3230-3237. (doi:10.1016/j.molimm.2008.02.020). (PMID:18403020).

Record type: Article


In the present report we have determined the molecular mechanisms, which govern the expression of the human IL-10 gene when induced by the glucocorticoid Methyl-Prednisolone (MP). Treatment of cells with MP at 10(-6) M will readily induce IL-10 in CD19+ primary B cells and in a human B cell line. Analysis of the IL-10 promoter showed a robust 18-fold induction and demonstrated that a potential GRE motif was not required, while mutation of the -120 STAT-motif strongly reduced MP-induced trans-activation. A strong induction was also seen with a trimeric STAT-motif and over-expression of dominant-negative STAT3 could block MP induction of IL-10 mRNA. Finally, MP treatment induced binding of STAT3 to the promoter as shown by gelshift, supershift and by chromatin-immunoprecipitation. These data show that glucocorticoid-induced expression of the IL-10 gene is mediated by the transcription factor STAT3.

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Published date: June 2008


Local EPrints ID: 185833
ISSN: 0161-5890
PURE UUID: 5a6a3004-33dd-4e69-b063-e7288a5b775d
ORCID for Karl J. Staples: ORCID iD

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Date deposited: 11 May 2011 10:16
Last modified: 18 Apr 2017 02:15

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Author: Claudia Unterberger
Author: Karl J. Staples ORCID iD
Author: Timothy Smallie
Author: Lynn Williams
Author: Brian Foxwell
Author: Annette Schaefer
Author: Bettina Kempkes
Author: T.P.J. Hofer
Author: Max Koeppel
Author: Marion Lohrum
Author: Henk Stunnenberg
Author: Marion Frankenberger
Author: Loems Ziegler-Heitbrock

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