The University of Southampton
University of Southampton Institutional Repository

A combined zinc/cadmium sensor and zinc/cadmium export regulator in a heavy metal pump

A combined zinc/cadmium sensor and zinc/cadmium export regulator in a heavy metal pump
A combined zinc/cadmium sensor and zinc/cadmium export regulator in a heavy metal pump
Heavy metal pumps (P1B-ATPases) are important for cellular heavy metal homeostasis. AtHMA4, an Arabidopsis thaliana heavy metal pump of importance for plant Zn2+ nutrition, has an extended C-terminal domain containing 13 cysteine pairs and a terminal stretch of 11 histidines. Using a novel size-exclusion chromatography, inductively coupled plasma mass spectrometry approach we report that the C-terminal domain of AtHMA4 is a high affinity Zn2+ and Cd2+ chelator with capacity to bind 10 Zn2+ ions per C terminus. When AtHMA4 is expressed in a Zn2+-sensitive zrc1 cot1 yeast strain, sequential removal of the histidine stretch and the cysteine pairs confers a gradual increase in Zn2+ and Cd2+ tolerance and lowered Zn2+ and Cd2+ content of transformed yeast cells. We conclude that the C-terminal domain of AtHMA4 serves a dual role as Zn2+ and Cd2+ chelator (sensor) and as a regulator of the efficiency of Zn2+ and Cd2+ export. The identification of a post-translational handle on Zn2+ and Cd2+ transport efficiency opens new perspectives for regulation of Zn2+ nutrition and tolerance in eukaryotes.

atpases, plant, plasma membrane, transport metals, zinc, cadmium, p-type pump, regulatory domain
0021-9258
31243-31252
Baekgaard, Lone
17446bd4-f3e9-420e-8ea7-2ab1764ae2d4
Mikkelsen, Maria D.
0fcdc09b-115e-4405-8e0b-00b4cd2662af
Sorensen, Danny M.
0a57d829-4a92-4a49-9cec-bb08408a5e15
Hegelund, Josefine N.
b6140607-c7c2-4545-bde8-14c0d29f9cc3
Persson, Daniel P.
c6d3dd61-8524-453d-849d-ffa7bbafc403
Mills, Rebecca F.
dec4afc8-be3b-4517-9038-c3c3e8b71b35
Yang, Zhang
de0b1548-4044-43f6-bc16-cd21cea24af5
Husted, Søren
9cba7f0e-b029-4f5d-ac0d-d58d0f20e274
Andersen, Jens Peter
1549d5d4-08e4-4ab3-be93-fb5df8741fd9
Buch-Pedersen, Morten J.
cd380e3f-40ec-4a63-90c9-8462736f6a98
Schjoerring, Jan K.
ab7fb9f6-31f6-4bdd-96d2-0aa8bd09a350
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Palmgren, Michael G.
397acbcb-5064-4af1-88c4-a2d2b89a9401
Baekgaard, Lone
17446bd4-f3e9-420e-8ea7-2ab1764ae2d4
Mikkelsen, Maria D.
0fcdc09b-115e-4405-8e0b-00b4cd2662af
Sorensen, Danny M.
0a57d829-4a92-4a49-9cec-bb08408a5e15
Hegelund, Josefine N.
b6140607-c7c2-4545-bde8-14c0d29f9cc3
Persson, Daniel P.
c6d3dd61-8524-453d-849d-ffa7bbafc403
Mills, Rebecca F.
dec4afc8-be3b-4517-9038-c3c3e8b71b35
Yang, Zhang
de0b1548-4044-43f6-bc16-cd21cea24af5
Husted, Søren
9cba7f0e-b029-4f5d-ac0d-d58d0f20e274
Andersen, Jens Peter
1549d5d4-08e4-4ab3-be93-fb5df8741fd9
Buch-Pedersen, Morten J.
cd380e3f-40ec-4a63-90c9-8462736f6a98
Schjoerring, Jan K.
ab7fb9f6-31f6-4bdd-96d2-0aa8bd09a350
Williams, Lorraine E.
79ee1856-3732-492b-8ac5-239749c85d9e
Palmgren, Michael G.
397acbcb-5064-4af1-88c4-a2d2b89a9401

Baekgaard, Lone, Mikkelsen, Maria D., Sorensen, Danny M., Hegelund, Josefine N., Persson, Daniel P., Mills, Rebecca F., Yang, Zhang, Husted, Søren, Andersen, Jens Peter, Buch-Pedersen, Morten J., Schjoerring, Jan K., Williams, Lorraine E. and Palmgren, Michael G. (2010) A combined zinc/cadmium sensor and zinc/cadmium export regulator in a heavy metal pump. The Journal of Biological Chemistry, 285 (41), 31243-31252. (doi:10.1074/jbc.M110.111260). (PMID:20650903)

Record type: Article

Abstract

Heavy metal pumps (P1B-ATPases) are important for cellular heavy metal homeostasis. AtHMA4, an Arabidopsis thaliana heavy metal pump of importance for plant Zn2+ nutrition, has an extended C-terminal domain containing 13 cysteine pairs and a terminal stretch of 11 histidines. Using a novel size-exclusion chromatography, inductively coupled plasma mass spectrometry approach we report that the C-terminal domain of AtHMA4 is a high affinity Zn2+ and Cd2+ chelator with capacity to bind 10 Zn2+ ions per C terminus. When AtHMA4 is expressed in a Zn2+-sensitive zrc1 cot1 yeast strain, sequential removal of the histidine stretch and the cysteine pairs confers a gradual increase in Zn2+ and Cd2+ tolerance and lowered Zn2+ and Cd2+ content of transformed yeast cells. We conclude that the C-terminal domain of AtHMA4 serves a dual role as Zn2+ and Cd2+ chelator (sensor) and as a regulator of the efficiency of Zn2+ and Cd2+ export. The identification of a post-translational handle on Zn2+ and Cd2+ transport efficiency opens new perspectives for regulation of Zn2+ nutrition and tolerance in eukaryotes.

Full text not available from this repository.

More information

Published date: 8 October 2010
Keywords: atpases, plant, plasma membrane, transport metals, zinc, cadmium, p-type pump, regulatory domain

Identifiers

Local EPrints ID: 186097
URI: https://eprints.soton.ac.uk/id/eprint/186097
ISSN: 0021-9258
PURE UUID: 67c3dc6c-8cb3-469a-9771-9f77fff27167

Catalogue record

Date deposited: 12 May 2011 10:43
Last modified: 18 Jul 2017 11:48

Export record

Altmetrics

Contributors

Author: Lone Baekgaard
Author: Maria D. Mikkelsen
Author: Danny M. Sorensen
Author: Josefine N. Hegelund
Author: Daniel P. Persson
Author: Rebecca F. Mills
Author: Zhang Yang
Author: Søren Husted
Author: Jens Peter Andersen
Author: Morten J. Buch-Pedersen
Author: Jan K. Schjoerring
Author: Michael G. Palmgren

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×