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A mannose-sensitive haemagglutinin (MSHA)-like pilus promotes attachment of Pseudoalteromonas tunicata cells to the surface of the green alga Ulva australis

A mannose-sensitive haemagglutinin (MSHA)-like pilus promotes attachment of Pseudoalteromonas tunicata cells to the surface of the green alga Ulva australis
A mannose-sensitive haemagglutinin (MSHA)-like pilus promotes attachment of Pseudoalteromonas tunicata cells to the surface of the green alga Ulva australis
This study demonstrates that attachment of the marine bacterium Pseudoalteromonas tunicata to the cellulose-containing surface of the green alga Ulva australis is mediated by a mannose-sensitive haemagglutinin (MSHA-like) pilus. We have identified an MSHA pilus biogenesis gene locus in P. tunicata, termed mshI1I2JKLMNEGFBACDOPQ, which shows significant homology, with respect to its genetic characteristics and organization, to the MSHA pilus biogenesis gene locus of Vibrio cholerae. Electron microscopy studies revealed that P. tunicata wild-type cells express flexible pili peritrichously arranged on the cell surface. A P. tunicata mutant (SM5) with a transposon insertion in the mshJ region displayed a non-piliated phenotype. Using SM5, it has been demonstrated that the MSHA pilus promotes attachment of P. tunicata wild-type cells in polystyrene microtitre plates, as well as to microcrystalline cellulose and to the living surface of U. australis. P. tunicata also demonstrated increased pilus production in response to cellulose and its monomer constituent cellobiose. The MSHA pilus thus functions as a determinant of attachment in P. tunicata, and it is proposed that an understanding of surface sensing mechanisms displayed by P. tunicata will provide insight into specific ecological interactions that occur between this bacterium and higher marine organisms
1350-0872
2875-2883
Dalisay, Doralyn S
33913674-eb0a-4e1a-9566-4d92c30335ad
Webb, Jeremy S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Scheffel, Andre
a61e1623-8208-4b23-b8ff-07cb7f17edf4
Svenson, Charles
4a96ecf9-4ba6-47d3-90f8-a3e75e509b64
James, Sally
56bebdac-9ab9-4113-a55d-8916da07df58
Holmstrom, Carola
33e36645-05ec-4c0b-af9b-f10257ed1b21
Egan, Suhelen
ed013d4f-f8c5-40b2-b3e1-caec2a6fb1fc
Kjelleberg, Staffan
043b66b5-130c-42f2-99b3-ec3eecf3248e
Dalisay, Doralyn S, Webb, Jeremy S., Scheffel, Andre, Svenson, Charles, James, Sally, Holmstrom, Carola, Egan, Suhelen and Kjelleberg, Staffan (2006) A mannose-sensitive haemagglutinin (MSHA)-like pilus promotes attachment of Pseudoalteromonas tunicata cells to the surface of the green alga Ulva australis Microbiology, 152, (10), pp. 2875-2883. (doi:10.1099/mic.0.29158-0). (PMID:17005969).

Dalisay, Doralyn S, Webb, Jeremy S., Scheffel, Andre, Svenson, Charles, James, Sally, Holmstrom, Carola, Egan, Suhelen and Kjelleberg, Staffan (2006) A mannose-sensitive haemagglutinin (MSHA)-like pilus promotes attachment of Pseudoalteromonas tunicata cells to the surface of the green alga Ulva australis Microbiology, 152, (10), pp. 2875-2883. (doi:10.1099/mic.0.29158-0). (PMID:17005969).

Record type: Article

Abstract

This study demonstrates that attachment of the marine bacterium Pseudoalteromonas tunicata to the cellulose-containing surface of the green alga Ulva australis is mediated by a mannose-sensitive haemagglutinin (MSHA-like) pilus. We have identified an MSHA pilus biogenesis gene locus in P. tunicata, termed mshI1I2JKLMNEGFBACDOPQ, which shows significant homology, with respect to its genetic characteristics and organization, to the MSHA pilus biogenesis gene locus of Vibrio cholerae. Electron microscopy studies revealed that P. tunicata wild-type cells express flexible pili peritrichously arranged on the cell surface. A P. tunicata mutant (SM5) with a transposon insertion in the mshJ region displayed a non-piliated phenotype. Using SM5, it has been demonstrated that the MSHA pilus promotes attachment of P. tunicata wild-type cells in polystyrene microtitre plates, as well as to microcrystalline cellulose and to the living surface of U. australis. P. tunicata also demonstrated increased pilus production in response to cellulose and its monomer constituent cellobiose. The MSHA pilus thus functions as a determinant of attachment in P. tunicata, and it is proposed that an understanding of surface sensing mechanisms displayed by P. tunicata will provide insight into specific ecological interactions that occur between this bacterium and higher marine organisms

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Published date: October 2006

Identifiers

Local EPrints ID: 186813
URI: http://eprints.soton.ac.uk/id/eprint/186813
ISSN: 1350-0872
PURE UUID: fb03ec2c-0f9c-46d9-a8e2-486e3ad469b1
ORCID for Jeremy S. Webb: ORCID iD orcid.org/0000-0003-2068-8589

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Date deposited: 18 May 2011 14:27
Last modified: 18 Jul 2017 11:46

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Contributors

Author: Doralyn S Dalisay
Author: Jeremy S. Webb ORCID iD
Author: Andre Scheffel
Author: Charles Svenson
Author: Sally James
Author: Carola Holmstrom
Author: Suhelen Egan
Author: Staffan Kjelleberg

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