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A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms

A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms
A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms
Pseudomonas aeruginosa produces extracellular DNA which functions as a cell-to-cell interconnecting matrix component in biofilms. Comparison of extracellular DNA and chromosomal DNA by the use of polymerase chain reaction and Southern analysis suggested that the extracellular DNA is similar to whole-genome DNA. Evidence that the extracellular DNA in P. aeruginosa biofilms and cultures is generated via lysis of a subpopulation of the bacteria was obtained through experiments where extracellular ?-galactosidase released from lacZ-containing P. aeruginosa strains was assessed. Experiments with the wild type and lasIrhlI, pqsA, pqsL and fliMpilA mutants indicated that the extracellular DNA is generated via a mechanism which is dependent on acyl homoserine lactone and Pseudomonas quinolone signalling, as well as on flagella and type IV pili. Microscopic investigation of flow chamber-grown wild-type P. aeruginosa biofilms stained with different DNA stains suggested that the extracellular DNA is located primarily in the stalks of mushroom-shaped multicellular structures, with a high concentration especially in the outer part of the stalks forming a border between the stalk-forming bacteria and the cap-forming bacteria. Biofilms formed by lasIrhlI, pqsA and fliMpilA mutants contained less extracellular DNA than biofilms formed by the wild type, and the mutant biofilms were more susceptible to treatment with sodium dodecyl sulphate than the wild-type biofilm
0950-382X
1114-1128
Allesen-Holm, Marie
7e02603a-e5c1-41c4-8c7d-1567141bbf3a
Barken, Kim Bundvig
81a3eedd-043a-4497-bc84-cdcadcf5a6a5
Yang, Liang
0aebf006-de9e-49e4-885d-ba26cc5b08e6
Klausen, Mikkel
bf0bdb05-53c8-486a-8c66-dd373c61e944
Webb, Jeremy S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Kjelleberg, Staffan
043b66b5-130c-42f2-99b3-ec3eecf3248e
Molin, Soren
8cd5bf22-034a-4962-9ea0-8a9ae4dda335
Givskov, Michael
44e11bca-55f6-4351-ba38-03e8233bc386
Tolker-Nielsen, Tim
4fcb7265-8c43-4118-aef1-c35dbe172740
Allesen-Holm, Marie
7e02603a-e5c1-41c4-8c7d-1567141bbf3a
Barken, Kim Bundvig
81a3eedd-043a-4497-bc84-cdcadcf5a6a5
Yang, Liang
0aebf006-de9e-49e4-885d-ba26cc5b08e6
Klausen, Mikkel
bf0bdb05-53c8-486a-8c66-dd373c61e944
Webb, Jeremy S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Kjelleberg, Staffan
043b66b5-130c-42f2-99b3-ec3eecf3248e
Molin, Soren
8cd5bf22-034a-4962-9ea0-8a9ae4dda335
Givskov, Michael
44e11bca-55f6-4351-ba38-03e8233bc386
Tolker-Nielsen, Tim
4fcb7265-8c43-4118-aef1-c35dbe172740

Allesen-Holm, Marie, Barken, Kim Bundvig, Yang, Liang, Klausen, Mikkel, Webb, Jeremy S., Kjelleberg, Staffan, Molin, Soren, Givskov, Michael and Tolker-Nielsen, Tim (2006) A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms. Molecular Microbiology, 59 (4), 1114-1128. (doi:10.1111/j.1365-2958.2005.05008.x).

Record type: Article

Abstract

Pseudomonas aeruginosa produces extracellular DNA which functions as a cell-to-cell interconnecting matrix component in biofilms. Comparison of extracellular DNA and chromosomal DNA by the use of polymerase chain reaction and Southern analysis suggested that the extracellular DNA is similar to whole-genome DNA. Evidence that the extracellular DNA in P. aeruginosa biofilms and cultures is generated via lysis of a subpopulation of the bacteria was obtained through experiments where extracellular ?-galactosidase released from lacZ-containing P. aeruginosa strains was assessed. Experiments with the wild type and lasIrhlI, pqsA, pqsL and fliMpilA mutants indicated that the extracellular DNA is generated via a mechanism which is dependent on acyl homoserine lactone and Pseudomonas quinolone signalling, as well as on flagella and type IV pili. Microscopic investigation of flow chamber-grown wild-type P. aeruginosa biofilms stained with different DNA stains suggested that the extracellular DNA is located primarily in the stalks of mushroom-shaped multicellular structures, with a high concentration especially in the outer part of the stalks forming a border between the stalk-forming bacteria and the cap-forming bacteria. Biofilms formed by lasIrhlI, pqsA and fliMpilA mutants contained less extracellular DNA than biofilms formed by the wild type, and the mutant biofilms were more susceptible to treatment with sodium dodecyl sulphate than the wild-type biofilm

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Published date: February 2006

Identifiers

Local EPrints ID: 186823
URI: https://eprints.soton.ac.uk/id/eprint/186823
ISSN: 0950-382X
PURE UUID: 0119743f-598f-4695-9b1e-9c09e90f60e1
ORCID for Jeremy S. Webb: ORCID iD orcid.org/0000-0003-2068-8589

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Date deposited: 18 May 2011 13:43
Last modified: 06 Jun 2018 12:39

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Contributors

Author: Marie Allesen-Holm
Author: Kim Bundvig Barken
Author: Liang Yang
Author: Mikkel Klausen
Author: Jeremy S. Webb ORCID iD
Author: Staffan Kjelleberg
Author: Soren Molin
Author: Michael Givskov
Author: Tim Tolker-Nielsen

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