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Self-taken pharyngeal and rectal swabs are appropriate for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in asymptomatic men who have sex with men

Self-taken pharyngeal and rectal swabs are appropriate for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in asymptomatic men who have sex with men
Self-taken pharyngeal and rectal swabs are appropriate for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in asymptomatic men who have sex with men
Introduction: Self-taken specimens from men who have sex with men (MSM) could be important in reducing high levels of demand on sexual health services. The performance of self-taken specimens for the detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) from both pharyngeal and rectal sites in asymptomatic MSM was assessed.

Methods: MSM were examined according to clinic protocol: a rectal and pharyngeal swab for GC culture and a rectal swab for the CT strand displacement assay. An extra set of nurse-taken and self-taken pharyngeal and rectal specimens were also requested and were tested using the Aptima Combo 2 assay and the result compared with the routine clinic result, which was considered the gold standard.

Results: A total of 272 MSM was recruited and the sensitivity and specificity of nurse-taken and patient-taken swabs, respectively, was as follows: rectal GC: 94.9% and 90.1% (nurse); 92.3% and 87.9% (patient); pharyngeal GC: 88.2% and 91.8% (nurse); 100% and 87.8% (patient); rectal CT: 80.0% and 99.6% (nurse); 91.4% and 98.2% (patient). No significant difference in sensitivity or specificity was observed between the nurse-taken and the patient-taken rectal swabs for either GC or CT. For the detection of GC from the pharynx, comparable sensitivities were achieved between nurse-taken and patient-taken swabs (p?=?0.5); however, a significant difference in specificity was observed (p?=?0.006). This was due to a higher number of false GC-positive self-taken pharyngeal swabs from patients with high rates (90.9%; 10/11) of confirmed concurrent GC infection in different anatomical sites.

Conclusions: MSM are able to collect self-taken rectal and pharyngeal swabs that are comparable to those taken by clinicians.

1368-4973
488-492
Alexander, S.
10077d9d-c2f0-4636-ab9e-ba55c8c5a0da
Ison, C.
19a355e8-83ba-466f-a43c-172b53254c9f
Parry, J.
8785ff22-4f4a-41a0-9735-5152c3dd3b64
Llewellyn, C.
867b1d97-fdd2-4230-84a5-36db0ab6361f
Wayal, S.
fbbb3b1d-5c2c-4252-80d9-7058914b6728
Richardson, D.
7374f16e-f868-426e-9678-555e9666312b
Phillips, A.
3f6d00b5-fb3c-423b-976d-83f22a2dc47b
Smith, H.
cc42a332-71ec-436f-8207-9151275a92d8
Fisher, M.
1ba6a9e9-9021-4c95-8423-4f04c52b6f9e
Brighton Home Sampling Kits Steering Group.
Alexander, S.
10077d9d-c2f0-4636-ab9e-ba55c8c5a0da
Ison, C.
19a355e8-83ba-466f-a43c-172b53254c9f
Parry, J.
8785ff22-4f4a-41a0-9735-5152c3dd3b64
Llewellyn, C.
867b1d97-fdd2-4230-84a5-36db0ab6361f
Wayal, S.
fbbb3b1d-5c2c-4252-80d9-7058914b6728
Richardson, D.
7374f16e-f868-426e-9678-555e9666312b
Phillips, A.
3f6d00b5-fb3c-423b-976d-83f22a2dc47b
Smith, H.
cc42a332-71ec-436f-8207-9151275a92d8
Fisher, M.
1ba6a9e9-9021-4c95-8423-4f04c52b6f9e

Alexander, S., Ison, C., Parry, J., Llewellyn, C., Wayal, S., Richardson, D., Phillips, A., Smith, H. and Fisher, M. , Brighton Home Sampling Kits Steering Group. (2008) Self-taken pharyngeal and rectal swabs are appropriate for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in asymptomatic men who have sex with men. Sexually Transmitted Infections, 84 (6), 488-492. (doi:10.1136/sti.2008.031443). (PMID:19028953)

Record type: Article

Abstract

Introduction: Self-taken specimens from men who have sex with men (MSM) could be important in reducing high levels of demand on sexual health services. The performance of self-taken specimens for the detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) from both pharyngeal and rectal sites in asymptomatic MSM was assessed.

Methods: MSM were examined according to clinic protocol: a rectal and pharyngeal swab for GC culture and a rectal swab for the CT strand displacement assay. An extra set of nurse-taken and self-taken pharyngeal and rectal specimens were also requested and were tested using the Aptima Combo 2 assay and the result compared with the routine clinic result, which was considered the gold standard.

Results: A total of 272 MSM was recruited and the sensitivity and specificity of nurse-taken and patient-taken swabs, respectively, was as follows: rectal GC: 94.9% and 90.1% (nurse); 92.3% and 87.9% (patient); pharyngeal GC: 88.2% and 91.8% (nurse); 100% and 87.8% (patient); rectal CT: 80.0% and 99.6% (nurse); 91.4% and 98.2% (patient). No significant difference in sensitivity or specificity was observed between the nurse-taken and the patient-taken rectal swabs for either GC or CT. For the detection of GC from the pharynx, comparable sensitivities were achieved between nurse-taken and patient-taken swabs (p?=?0.5); however, a significant difference in specificity was observed (p?=?0.006). This was due to a higher number of false GC-positive self-taken pharyngeal swabs from patients with high rates (90.9%; 10/11) of confirmed concurrent GC infection in different anatomical sites.

Conclusions: MSM are able to collect self-taken rectal and pharyngeal swabs that are comparable to those taken by clinicians.

Full text not available from this repository.

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Published date: November 2008

Identifiers

Local EPrints ID: 187353
URI: https://eprints.soton.ac.uk/id/eprint/187353
ISSN: 1368-4973
PURE UUID: 7620c4fa-3f92-4b13-8297-baaf4a6628b3

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Date deposited: 17 May 2011 13:53
Last modified: 17 Oct 2018 16:31

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