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Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compound

Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compound
Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compound
Real-time PCR was used to measure changes in transcript abundance of genes encoding important immune proteins, namely prophenoloxidase (proPO gene), ?-1,3-glucan binding protein (?GBP gene) and a 12.2 kDa antimicrobial peptide (amp gene) in post-larval stage VI (PLVI) juveniles of the European lobster, Homarus gammarus. Gene expression was studied in both healthy PLVI and following single or repeat exposure to a range of compounds claimed to induce immune reactivity. A single acute (3-h) exposure to any of the tested stimulants did not produce a significant increase in expression of either the proPO or ?GBP genes, measured 6 h after stimulation. However, there were a small sub-group of positive responders, identified mainly from ?GBP expression, within the experimental groups stimulated with either a ?-1,3-glucan or an alginate. There was also no significant increase in the expression of any of the three genes tested 24 h after repeated weekly (3-h) exposures to a either the ?-1,3-glucan or the alginate over the longer (36-day) period. The results do show that amp is expressed at an extremely high level compared to proPO or ?GBP in healthy animals and a significant correlation was found between the expression of proPO and both ?GBP and amp, irrespective of whether or not the larvae were stimulated. None of the immune stimulated compounds improved survival of PLVI challenged with the opportunistic pathogen, Listonella anguillarum, or the lobster pathogen, Aerococcus viridans var. homari. Thus, we found no evidence to support recent claims that immunity and disease resistance can be primed or promoted within a given population of crustaceans or that these animals exhibit functional immune memory to some soluble immune elicitors.
0161-5890
443-450
Hauton, C.
7706f6ba-4497-42b2-8c6d-00df81676331
Brockton, V.
61eca502-9c6c-4be5-bf1b-2b1cad383294
Smith, V.J.
7dc3f0fa-e744-4b55-b852-7c8af4abdaa3
Hauton, C.
7706f6ba-4497-42b2-8c6d-00df81676331
Brockton, V.
61eca502-9c6c-4be5-bf1b-2b1cad383294
Smith, V.J.
7dc3f0fa-e744-4b55-b852-7c8af4abdaa3

Hauton, C., Brockton, V. and Smith, V.J. (2007) Changes in immune gene expression and resistance to bacterial infection in lobster (Homarus gammarus) post-larval stage VI following acute or chronic exposure to immune stimulating compound. Molecular Immunology, 44 (4), 443-450. (doi:10.1016/j.molimm.2006.02.018).

Record type: Article

Abstract

Real-time PCR was used to measure changes in transcript abundance of genes encoding important immune proteins, namely prophenoloxidase (proPO gene), ?-1,3-glucan binding protein (?GBP gene) and a 12.2 kDa antimicrobial peptide (amp gene) in post-larval stage VI (PLVI) juveniles of the European lobster, Homarus gammarus. Gene expression was studied in both healthy PLVI and following single or repeat exposure to a range of compounds claimed to induce immune reactivity. A single acute (3-h) exposure to any of the tested stimulants did not produce a significant increase in expression of either the proPO or ?GBP genes, measured 6 h after stimulation. However, there were a small sub-group of positive responders, identified mainly from ?GBP expression, within the experimental groups stimulated with either a ?-1,3-glucan or an alginate. There was also no significant increase in the expression of any of the three genes tested 24 h after repeated weekly (3-h) exposures to a either the ?-1,3-glucan or the alginate over the longer (36-day) period. The results do show that amp is expressed at an extremely high level compared to proPO or ?GBP in healthy animals and a significant correlation was found between the expression of proPO and both ?GBP and amp, irrespective of whether or not the larvae were stimulated. None of the immune stimulated compounds improved survival of PLVI challenged with the opportunistic pathogen, Listonella anguillarum, or the lobster pathogen, Aerococcus viridans var. homari. Thus, we found no evidence to support recent claims that immunity and disease resistance can be primed or promoted within a given population of crustaceans or that these animals exhibit functional immune memory to some soluble immune elicitors.

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Submitted date: 8 December 2005
Accepted/In Press date: 8 December 2005
Published date: 2007

Identifiers

Local EPrints ID: 188117
URI: http://eprints.soton.ac.uk/id/eprint/188117
ISSN: 0161-5890
PURE UUID: 57c47454-20aa-44b4-bee2-58e3495eb5f3
ORCID for C. Hauton: ORCID iD orcid.org/0000-0002-2313-4226

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Date deposited: 20 May 2011 10:34
Last modified: 08 Jan 2022 02:44

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Contributors

Author: C. Hauton ORCID iD
Author: V. Brockton
Author: V.J. Smith

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