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Windows to cell function and dysfunction: signatures written in the boundary layers

Windows to cell function and dysfunction: signatures written in the boundary layers
Windows to cell function and dysfunction: signatures written in the boundary layers
The medium surrounding cells either in culture or in tissues contains a chemical mix varying with cell state. As solutes move in and out of the cytoplasmic compartment they set up characteristic signatures in the cellular boundary layers. These layers are complex physical and chemical environments the profiles of which reflect cell physiology and provide conduits for intercellular messaging. Here we review some of the most relevant characteristics of the extracellular/intercellular space. Our initial focus is primarily on cultured cells but we extend our consideration to the far more complex environment of tissues, and discuss how chemical signatures in the boundary layer can or may affect cell function. Critical to the entire essay are the methods used, or being developed, to monitor chemical profiles in the boundary layers. We review recent developments in ultramicro electrochemical sensors and tailored optical reporters suitable for the task in hand.
chemical profiles, electrochemical sensing, imaging, intercellular space, unstirred layers
0265-9247
514-523
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Collis, Leon P.
66f825a3-f4a7-4e85-a1ca-ee16497b8a81
Messerli, Mark A.
94a8bd34-95d7-4a52-b4d8-1ccded0e8fe7
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Collis, Leon P.
66f825a3-f4a7-4e85-a1ca-ee16497b8a81
Messerli, Mark A.
94a8bd34-95d7-4a52-b4d8-1ccded0e8fe7

Smith, Peter J.S., Collis, Leon P. and Messerli, Mark A. (2010) Windows to cell function and dysfunction: signatures written in the boundary layers. BioEssays, 32 (6), 514-523. (doi:10.1002/bies.200900173). (PMID:20486138)

Record type: Article

Abstract

The medium surrounding cells either in culture or in tissues contains a chemical mix varying with cell state. As solutes move in and out of the cytoplasmic compartment they set up characteristic signatures in the cellular boundary layers. These layers are complex physical and chemical environments the profiles of which reflect cell physiology and provide conduits for intercellular messaging. Here we review some of the most relevant characteristics of the extracellular/intercellular space. Our initial focus is primarily on cultured cells but we extend our consideration to the far more complex environment of tissues, and discuss how chemical signatures in the boundary layer can or may affect cell function. Critical to the entire essay are the methods used, or being developed, to monitor chemical profiles in the boundary layers. We review recent developments in ultramicro electrochemical sensors and tailored optical reporters suitable for the task in hand.

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More information

e-pub ahead of print date: 18 May 2010
Published date: June 2010
Keywords: chemical profiles, electrochemical sensing, imaging, intercellular space, unstirred layers
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 188743
URI: http://eprints.soton.ac.uk/id/eprint/188743
ISSN: 0265-9247
PURE UUID: e3dcda47-b730-43f7-b5a6-a87fd16a4f64
ORCID for Peter J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853

Catalogue record

Date deposited: 27 May 2011 15:58
Last modified: 20 Jul 2019 00:44

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