The University of Southampton
University of Southampton Institutional Repository

Dielectrophoretic assembly of insulinoma cells and fluorescent nanosensors into three-dimensional pseudo-islet constructs

Dielectrophoretic assembly of insulinoma cells and fluorescent nanosensors into three-dimensional pseudo-islet constructs
Dielectrophoretic assembly of insulinoma cells and fluorescent nanosensors into three-dimensional pseudo-islet constructs
Dielectrophoretic forces, generated by radio-frequency voltages applied to micromachined, transparent, indium tin oxide electrodes, have been used to condense suspensions of insulinoma cells (BETA-TC-6 and INS-1) into a 10times10 array of three-dimensional cell constructs. Some of these constructs, measuring ~150 mum in diameter, 120 mum in height and containing around 1000 cells, were of the same size and cell density as a typical islet of Langerhans. With the dielectrophoretic force maintained, these engineered cell constructs were able to withstand mechanical shock and fluid flow forces. Reproducibility of the process required knowledge of cellular dielectric properties, in terms of membrane capacitance and membrane conductance, which were obtained by electrorotation measurements. The ability to incorporate fluorescent nanosensors, as probes of cellular oxygen and pH levels, into these 'pseudo-islets' was also demonstrated. The footprint of the 10times10 array of cell constructs was compatible with that of a 1536 microtitre plate, and thus amenable to optical interrogation using automated plate reading equipment.
1751-8741
31-38
Pethig, R.
e58f40b8-85b0-4982-89a9-56d781eea024
Menachery, A.
141dcc97-491e-46fb-972b-a36dda1786d5
Heart, E.
a0e99eb3-233a-4e68-8258-20c170c82d03
Sanger, R.H.
ec3d4693-8abe-4cd0-89c0-02f44ebb056b
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Pethig, R.
e58f40b8-85b0-4982-89a9-56d781eea024
Menachery, A.
141dcc97-491e-46fb-972b-a36dda1786d5
Heart, E.
a0e99eb3-233a-4e68-8258-20c170c82d03
Sanger, R.H.
ec3d4693-8abe-4cd0-89c0-02f44ebb056b
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c

Pethig, R., Menachery, A., Heart, E., Sanger, R.H. and Smith, P.J.S. (2008) Dielectrophoretic assembly of insulinoma cells and fluorescent nanosensors into three-dimensional pseudo-islet constructs. IET Nanobiotechnology, 2 (2), 31-38. (doi:10.1049/iet-nbt:20070027).

Record type: Article

Abstract

Dielectrophoretic forces, generated by radio-frequency voltages applied to micromachined, transparent, indium tin oxide electrodes, have been used to condense suspensions of insulinoma cells (BETA-TC-6 and INS-1) into a 10times10 array of three-dimensional cell constructs. Some of these constructs, measuring ~150 mum in diameter, 120 mum in height and containing around 1000 cells, were of the same size and cell density as a typical islet of Langerhans. With the dielectrophoretic force maintained, these engineered cell constructs were able to withstand mechanical shock and fluid flow forces. Reproducibility of the process required knowledge of cellular dielectric properties, in terms of membrane capacitance and membrane conductance, which were obtained by electrorotation measurements. The ability to incorporate fluorescent nanosensors, as probes of cellular oxygen and pH levels, into these 'pseudo-islets' was also demonstrated. The footprint of the 10times10 array of cell constructs was compatible with that of a 1536 microtitre plate, and thus amenable to optical interrogation using automated plate reading equipment.

Text
Final_draft_NBT-2007-0027___Pethig_et_al__.pdf - Accepted Manuscript
Download (667kB)

More information

Published date: June 2008
Organisations: University of Southampton

Identifiers

Local EPrints ID: 188747
URI: http://eprints.soton.ac.uk/id/eprint/188747
ISSN: 1751-8741
PURE UUID: aad3c574-6dc3-42ee-9899-4f41c1b27642
ORCID for P.J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853

Catalogue record

Date deposited: 27 May 2011 15:49
Last modified: 03 Dec 2019 01:40

Export record

Altmetrics

Contributors

Author: R. Pethig
Author: A. Menachery
Author: E. Heart
Author: R.H. Sanger
Author: P.J.S. Smith ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×