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Characterization of optimized Na+ and Cl? liquid membranes for use with extracellular, self-referencing microelectrodes

Characterization of optimized Na+ and Cl? liquid membranes for use with extracellular, self-referencing microelectrodes
Characterization of optimized Na+ and Cl? liquid membranes for use with extracellular, self-referencing microelectrodes
Self-referencing with ion-selective microelectrodes (ISMs) is a useful approach for monitoring near-real-time ion flux near single cells and across epithelia. While ISMs for H+, Ca2+, and K+ have been optimized for use with self-referencing, ISMs for two other primary inorganic ions, Na+ and Cl-, have not. In this study, we have characterized ISMs based on three Na+ ionophores (I, VI, and X) and one Cl- ionophore to assess their suitability for use with self-referencing. ISMs constructed with Na+ ionophore VI have short response times (approximately 100 ms) but possess nearly an order of magnitude less selectivity for Na+ over K+ than ISMs constructed with Na+ ionophore X. The Na+ ionophore X mixture was enhanced to give it a shorter response time while not compromising its selectivity. A Cl(-)-selective microelectrode was constructed and characterized with superior anionic selectivity compared with previously reported Cl- ISMs used with self-referencing. This Cl(-)-selective microelectrode, however, has a relatively slow response time (approximately 3 s), thus requiring changes to the self-referencing protocol. Self-referencing with these ISMs will enable near-real-time ion flux measurements for Na+ and Cl-.
1618-2642
1355-1359
Messerli, Mark A.
94a8bd34-95d7-4a52-b4d8-1ccded0e8fe7
Kurtz, Ira
9da04fed-f31b-43d4-a2e4-d8b2e39ba43b
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Messerli, Mark A.
94a8bd34-95d7-4a52-b4d8-1ccded0e8fe7
Kurtz, Ira
9da04fed-f31b-43d4-a2e4-d8b2e39ba43b
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c

Messerli, Mark A., Kurtz, Ira and Smith, Peter J.S. (2008) Characterization of optimized Na+ and Cl? liquid membranes for use with extracellular, self-referencing microelectrodes. Analytical and Bioanalytical Chemistry, 390 (5), 1355-1359. (doi:10.1007/s00216-007-1804-z). (PMID:18193410)

Record type: Article

Abstract

Self-referencing with ion-selective microelectrodes (ISMs) is a useful approach for monitoring near-real-time ion flux near single cells and across epithelia. While ISMs for H+, Ca2+, and K+ have been optimized for use with self-referencing, ISMs for two other primary inorganic ions, Na+ and Cl-, have not. In this study, we have characterized ISMs based on three Na+ ionophores (I, VI, and X) and one Cl- ionophore to assess their suitability for use with self-referencing. ISMs constructed with Na+ ionophore VI have short response times (approximately 100 ms) but possess nearly an order of magnitude less selectivity for Na+ over K+ than ISMs constructed with Na+ ionophore X. The Na+ ionophore X mixture was enhanced to give it a shorter response time while not compromising its selectivity. A Cl(-)-selective microelectrode was constructed and characterized with superior anionic selectivity compared with previously reported Cl- ISMs used with self-referencing. This Cl(-)-selective microelectrode, however, has a relatively slow response time (approximately 3 s), thus requiring changes to the self-referencing protocol. Self-referencing with these ISMs will enable near-real-time ion flux measurements for Na+ and Cl-.

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Published date: March 2008
Organisations: University of Southampton

Identifiers

Local EPrints ID: 188773
URI: http://eprints.soton.ac.uk/id/eprint/188773
ISSN: 1618-2642
PURE UUID: ce1af21b-99c8-4365-9c3b-056f3305afa0
ORCID for Peter J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853

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Date deposited: 27 May 2011 14:39
Last modified: 29 Oct 2019 01:42

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Author: Mark A. Messerli
Author: Ira Kurtz

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