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Determination of single-cell oxygen consumption with impedance feedback for control of sample?probe separation

Determination of single-cell oxygen consumption with impedance feedback for control of sample?probe separation
Determination of single-cell oxygen consumption with impedance feedback for control of sample?probe separation
The ability to measure chemical gradients surrounding single cells provides novel insights into several areas of cell dynamics--particularly metabolism. Detection of metabolic oxygen consumption can be achieved from a single mammalian cell using a modulated amperometric sensor in a self-referencing mode. To date, however, apart from visual cues, we do not have a reliable and cell-compatible method for determining and stabilizing the position of such probes. In this paper, we report on having successfully measured the increase in the uncompensated resistance of an electrochemical cell upon approach to single, living, biological cells, while simultaneously measuring the metabolic oxygen consumption. This was accomplished by applying an ac and a dc excitation signal to the electrode. The applied ac waveform was a 100-kHz sine wave with an amplitude of 10 mV rms, while the dc voltage applied was -600 mV. The two signals were shown not to interfere with one another. Furthermore, it is shown that the sample-probe distance can be measured for approach to single cells on the order of 10-15-microm diameter and 5-microm height, with 100-nm resolution
0003-2700
6999-7004
Osbourn, Damon M.
81f20c5e-35f8-45d8-bf17-46a645a636fb
Sanger, Richard H.
eb4dde62-3c95-4a17-ac97-aba2a2ae5baf
Smith, Peter J. S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Osbourn, Damon M.
81f20c5e-35f8-45d8-bf17-46a645a636fb
Sanger, Richard H.
eb4dde62-3c95-4a17-ac97-aba2a2ae5baf
Smith, Peter J. S.
003de469-9420-4f12-8f0e-8e8d76d28d6c

Osbourn, Damon M., Sanger, Richard H. and Smith, Peter J. S. (2005) Determination of single-cell oxygen consumption with impedance feedback for control of sample?probe separation. Analytical Chemistry, 77 (21), 6999-7004. (doi:10.1021/ac050326w). (PMID:16255601)

Record type: Article

Abstract

The ability to measure chemical gradients surrounding single cells provides novel insights into several areas of cell dynamics--particularly metabolism. Detection of metabolic oxygen consumption can be achieved from a single mammalian cell using a modulated amperometric sensor in a self-referencing mode. To date, however, apart from visual cues, we do not have a reliable and cell-compatible method for determining and stabilizing the position of such probes. In this paper, we report on having successfully measured the increase in the uncompensated resistance of an electrochemical cell upon approach to single, living, biological cells, while simultaneously measuring the metabolic oxygen consumption. This was accomplished by applying an ac and a dc excitation signal to the electrode. The applied ac waveform was a 100-kHz sine wave with an amplitude of 10 mV rms, while the dc voltage applied was -600 mV. The two signals were shown not to interfere with one another. Furthermore, it is shown that the sample-probe distance can be measured for approach to single cells on the order of 10-15-microm diameter and 5-microm height, with 100-nm resolution

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Published date: November 2005
Organisations: University of Southampton

Identifiers

Local EPrints ID: 188821
URI: http://eprints.soton.ac.uk/id/eprint/188821
ISSN: 0003-2700
PURE UUID: b1ef615d-98ae-4e0d-ae07-c31d109fce99
ORCID for Peter J. S. Smith: ORCID iD orcid.org/0000-0003-4400-6853

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Date deposited: 06 Jun 2011 08:35
Last modified: 19 Nov 2019 01:39

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Author: Damon M. Osbourn
Author: Richard H. Sanger

University divisions

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