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Recognition of GT mismatches by Vsr mismatch endonuclease

Fox, K.R., Allinson, S.L., Sahagun-Krause, H. and Brown, T. (2000) Recognition of GT mismatches by Vsr mismatch endonuclease Nucleic Acids Research, 28, (13), pp. 2535-2540. (doi:10.1093/nar/28.13.2535).

Record type: Article

Abstract

The Vsr mismatch endonuclease recognises the sequence CTWGG (W = A or T) in which the underlined thymine is paired with guanine and nicks the DNA backbone on the 5?-side of the mispaired thymine. By using base analogues of G and T we have explored the functional groups on the mismatch pair which are recognised by the enzyme. Removal of the thymine 5-methyl group causes a 60% reduction in activity, while removing the 2-amino group of guanine reduces cleavage by 90%. Placing 2-amino­purine or nebularine opposite T generates mis­matches which are cut at a much lower rate (0.1%). When either base is removed, generating a pseudoabasic site (1?,2?-dideoxyribose), the enzyme still produces site-specific cleavage, but at only 1% of the original rate. Although TT and CT mismatches at this position are cleaved at a low rate (~1%), mismatches with other bases (such as GA and AC) and Watson–Crick base pairs are not cleaved by the enzyme. There is also no cleavage when the mismatched T is replaced with difluorotoluene.

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More information

Published date: 1 July 2000
Keywords: escherichia-coli k-12, short patch repair, cytosine methylation, crystal-structure, dna duplex, gene, 5-methyl-cytosine, difluorotoluene, cleavage, adenine

Identifiers

Local EPrints ID: 18926
URI: http://eprints.soton.ac.uk/id/eprint/18926
ISSN: 0305-1048
PURE UUID: b968ee00-4197-4730-94e5-7b28c815f88b
ORCID for K.R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 19 Jan 2006
Last modified: 17 Jul 2017 16:34

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Contributors

Author: K.R. Fox ORCID iD
Author: S.L. Allinson
Author: H. Sahagun-Krause
Author: T. Brown

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