Functional analysis of tenocytes gene expression in tendon fascicles subjected to cyclic tensile strain
Functional analysis of tenocytes gene expression in tendon fascicles subjected to cyclic tensile strain
Tenocytes are known to be mechanoresponsive and the present study tests the hypothesis that distinct mechanical stimulation regimes, associated with the short-term and extended application of cyclic tensile strain, alters the balance between anabolic and catabolic processes. Microarray technology has been used to provide a comprehensive analysis of alterations in gene expression within isolated tendon fascicles in response to cyclic tensile strain using a well-established model system. Isolated rat tail tendon fascicles were subjected to cyclic tensile strain (3% amplitude superimposed on a 2% static strain) for 1 or 24 hr. Messenger RNA expression level was assessed using Illumina microarray. The number of genes significantly altered in strained fascicles from the level of unstrained control fascicles was greater at 24 hr than 1 hr. The expression levels of many extracellular matrix components remained unchanged at both time points; however, a number of members of the matrix metalloproteinase (MMP) and a disintegrin and metalloproteinase with a thrombospondin (ADAMTS) families were significantly downregulated at 24 hr. Functional annotation revealed that upregulated genes were significantly associated with the regulation of transcription at 1 hr and translation at 24 hr. Downregulated genes were associated with inflammatory responses at 1 hr, and genes inhibited at 24 hr were significantly associated with cell apoptosis and a variety of metabolic functions. The present results suggest that the metabolic balance was shifted in favor of catabolism by the application of a small number of tensile strain cycles, whereas an extended number stimulates strong anti-catabolic effects.
mechanobiology, tendon, mechanotransduction, genomics, metabolism
434-444
Maeda, Eijiro
15867696-0c06-4c07-a637-fc8c603695d7
Fleischmann, Christina
e7adbc6d-338d-4646-898a-9c77afa0541d
Mein, Charles A.
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Shelton, Julia
b24906ad-130c-4f9b-a017-7423799ef81c
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871
December 2010
Maeda, Eijiro
15867696-0c06-4c07-a637-fc8c603695d7
Fleischmann, Christina
e7adbc6d-338d-4646-898a-9c77afa0541d
Mein, Charles A.
c1cc43af-6c34-47a4-a959-5105593bcbde
Shelton, Julia
b24906ad-130c-4f9b-a017-7423799ef81c
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871
Maeda, Eijiro, Fleischmann, Christina, Mein, Charles A., Shelton, Julia, Bader, Dan L. and Lee, David A.
(2010)
Functional analysis of tenocytes gene expression in tendon fascicles subjected to cyclic tensile strain.
Connective Tissue Research, 51 (6), .
(doi:10.3109/03008201003597056).
(PMID:20497018)
Abstract
Tenocytes are known to be mechanoresponsive and the present study tests the hypothesis that distinct mechanical stimulation regimes, associated with the short-term and extended application of cyclic tensile strain, alters the balance between anabolic and catabolic processes. Microarray technology has been used to provide a comprehensive analysis of alterations in gene expression within isolated tendon fascicles in response to cyclic tensile strain using a well-established model system. Isolated rat tail tendon fascicles were subjected to cyclic tensile strain (3% amplitude superimposed on a 2% static strain) for 1 or 24 hr. Messenger RNA expression level was assessed using Illumina microarray. The number of genes significantly altered in strained fascicles from the level of unstrained control fascicles was greater at 24 hr than 1 hr. The expression levels of many extracellular matrix components remained unchanged at both time points; however, a number of members of the matrix metalloproteinase (MMP) and a disintegrin and metalloproteinase with a thrombospondin (ADAMTS) families were significantly downregulated at 24 hr. Functional annotation revealed that upregulated genes were significantly associated with the regulation of transcription at 1 hr and translation at 24 hr. Downregulated genes were associated with inflammatory responses at 1 hr, and genes inhibited at 24 hr were significantly associated with cell apoptosis and a variety of metabolic functions. The present results suggest that the metabolic balance was shifted in favor of catabolism by the application of a small number of tensile strain cycles, whereas an extended number stimulates strong anti-catabolic effects.
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Published date: December 2010
Keywords:
mechanobiology, tendon, mechanotransduction, genomics, metabolism
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Local EPrints ID: 189395
URI: http://eprints.soton.ac.uk/id/eprint/189395
ISSN: 0300-8207
PURE UUID: c09526e7-264b-47da-9b16-228557789b75
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Date deposited: 03 Jun 2011 08:54
Last modified: 14 Mar 2024 03:35
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Contributors
Author:
Eijiro Maeda
Author:
Christina Fleischmann
Author:
Charles A. Mein
Author:
Julia Shelton
Author:
David A. Lee
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