A reaction–diffusion model to predict the influence of neo-matrix on the subsequent development of tissue-engineered cartilage
A reaction–diffusion model to predict the influence of neo-matrix on the subsequent development of tissue-engineered cartilage
Extracellular matrix (ECM) in chondrocytes-seeded agarose aggregates to form islands of matrix. These islands need to coalesce to develop functional cartilage. Hence, macroscopic properties are determined by transport and aggregation of macromolecules at the microscale, which varies temporally and spatially. This study evaluates the importance of the mutual interaction between matrix components and matrix development.
Fluorescence recovery after photobleaching measurements demonstrates that diffusivity depends on the presence and density of ECM. A reaction-diffusion model describing synthesis, transport and immobilisation of ECM predicts steep gradients in ECM around chondrocytes, resembling histology. Steric hindrance of diffusion by ECM is essential for the formation of these gradients. Finally, microscopic ECM concentration is linked with macroscopic mechanical properties. Construct softening is predicted when temporal and spatial variations in diffusivity are considered.
In conclusion, non-constant diffusion renders significant effects on both the microscopic ECM development and the macroscopic mechanical properties of developing tissue-engineered cartilage.
chondrocytes diffusion, frap, matrix development, tissue engineering
425-432
van Donkelaar, C.
714d0866-ca6d-4f75-9cbc-cd797a0e96fa
Chao, G.
49a46097-ac4a-43fa-91da-bc37887d2ae8
Bader, D.L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Oomens, C.
80d7f5db-e7ca-4db7-bae7-43a8c1cff615
May 2011
van Donkelaar, C.
714d0866-ca6d-4f75-9cbc-cd797a0e96fa
Chao, G.
49a46097-ac4a-43fa-91da-bc37887d2ae8
Bader, D.L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Oomens, C.
80d7f5db-e7ca-4db7-bae7-43a8c1cff615
van Donkelaar, C., Chao, G., Bader, D.L. and Oomens, C.
(2011)
A reaction–diffusion model to predict the influence of neo-matrix on the subsequent development of tissue-engineered cartilage.
Computer Methods in Biomechanics and Biomedical Engineering, 14 (5), .
(doi:10.1080/10255842.2011.554409).
(PMID:21516527)
Abstract
Extracellular matrix (ECM) in chondrocytes-seeded agarose aggregates to form islands of matrix. These islands need to coalesce to develop functional cartilage. Hence, macroscopic properties are determined by transport and aggregation of macromolecules at the microscale, which varies temporally and spatially. This study evaluates the importance of the mutual interaction between matrix components and matrix development.
Fluorescence recovery after photobleaching measurements demonstrates that diffusivity depends on the presence and density of ECM. A reaction-diffusion model describing synthesis, transport and immobilisation of ECM predicts steep gradients in ECM around chondrocytes, resembling histology. Steric hindrance of diffusion by ECM is essential for the formation of these gradients. Finally, microscopic ECM concentration is linked with macroscopic mechanical properties. Construct softening is predicted when temporal and spatial variations in diffusivity are considered.
In conclusion, non-constant diffusion renders significant effects on both the microscopic ECM development and the macroscopic mechanical properties of developing tissue-engineered cartilage.
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Published date: May 2011
Keywords:
chondrocytes diffusion, frap, matrix development, tissue engineering
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Local EPrints ID: 189407
URI: http://eprints.soton.ac.uk/id/eprint/189407
ISSN: 1025-5842
PURE UUID: d914913c-18a4-4d8f-b573-28ebaaf6aa97
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Date deposited: 02 Jun 2011 08:41
Last modified: 14 Mar 2024 03:35
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Author:
C. van Donkelaar
Author:
G. Chao
Author:
C. Oomens
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