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Expression of AtECA3 in tobacco modifies its responses to manganese, zinc and calcium

Expression of AtECA3 in tobacco modifies its responses to manganese, zinc and calcium
Expression of AtECA3 in tobacco modifies its responses to manganese, zinc and calcium
Mn- and Zn-deficiency or excess reduce plant growth and development. Engineering plants with enhanced metal tolerance and accumulation is a major goal in phytoremediation/phytostabilization. Moreover, improved growth under unfavourable mineral conditions contributes to better crop production. In this study, ECA3 cDNA from Arabidopsis thaliana, encoding a P2A-ATPase, was introduced into Nicotiana tabacum var. Xanthi, to examine its value for modifying responses to cations, primarily Mn, Zn and Ca. AtECA3 was ectopically expressed under the CaMV 35S promoter. Transgenic and wild-type plants were tested under hydroponic conditions for their responses to a range of metal exposures (low, moderate and high concentrations), and their tolerance and accumulation were evaluated. AtECA3 expression resulted in better growth of plants at moderate levels of Mn (2 ?M) in the medium and enhanced tolerance to high Mn (100 ?M). Transgenic plants were also more tolerant to Ca-deficiency conditions, although they showed no differences to wild-type with respect to overall Ca levels. Transgene expression did not produce one unique pattern of Mn and Zn accumulation but instead depended on the external concentration of the particular metal supplied. Thus the enhancement of plant productivity at moderate Mn levels and increased Mn tolerance at high (toxic) Mn supply, as well as the slight increase in Ca-deficiency tolerance seen in ECA3-transformed plants indicates that this gene could be useful in plant biotechnological strategies.

ateca3, manganese, p2A-atpase, transformation, zinc, calcium
0098-8472
202-209
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Mills, Rebecca Fay
a0a792e8-e2b3-468c-aa5c-2e273fd29f56
Trojanowska, Ewa
2f5ba5dc-ca08-4a4f-a6d5-f3374ab502cf
Williams, Lorraine Elizabeth
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b
Barabasz, Anna
0e750db4-b7da-4456-8465-426452b89a2b
Mills, Rebecca Fay
a0a792e8-e2b3-468c-aa5c-2e273fd29f56
Trojanowska, Ewa
2f5ba5dc-ca08-4a4f-a6d5-f3374ab502cf
Williams, Lorraine Elizabeth
79ee1856-3732-492b-8ac5-239749c85d9e
Antosiewicz, Danuta Maria
cbbcbcfe-2ce6-4737-9933-304d48a1590b

Barabasz, Anna, Mills, Rebecca Fay, Trojanowska, Ewa, Williams, Lorraine Elizabeth and Antosiewicz, Danuta Maria (2011) Expression of AtECA3 in tobacco modifies its responses to manganese, zinc and calcium. Environmental and Experimental Botany, 72 (2), 202-209. (doi:10.1016/j.envexpbot.2011.03.006).

Record type: Article

Abstract

Mn- and Zn-deficiency or excess reduce plant growth and development. Engineering plants with enhanced metal tolerance and accumulation is a major goal in phytoremediation/phytostabilization. Moreover, improved growth under unfavourable mineral conditions contributes to better crop production. In this study, ECA3 cDNA from Arabidopsis thaliana, encoding a P2A-ATPase, was introduced into Nicotiana tabacum var. Xanthi, to examine its value for modifying responses to cations, primarily Mn, Zn and Ca. AtECA3 was ectopically expressed under the CaMV 35S promoter. Transgenic and wild-type plants were tested under hydroponic conditions for their responses to a range of metal exposures (low, moderate and high concentrations), and their tolerance and accumulation were evaluated. AtECA3 expression resulted in better growth of plants at moderate levels of Mn (2 ?M) in the medium and enhanced tolerance to high Mn (100 ?M). Transgenic plants were also more tolerant to Ca-deficiency conditions, although they showed no differences to wild-type with respect to overall Ca levels. Transgene expression did not produce one unique pattern of Mn and Zn accumulation but instead depended on the external concentration of the particular metal supplied. Thus the enhancement of plant productivity at moderate Mn levels and increased Mn tolerance at high (toxic) Mn supply, as well as the slight increase in Ca-deficiency tolerance seen in ECA3-transformed plants indicates that this gene could be useful in plant biotechnological strategies.

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More information

Published date: September 2011
Keywords: ateca3, manganese, p2A-atpase, transformation, zinc, calcium

Identifiers

Local EPrints ID: 189429
URI: http://eprints.soton.ac.uk/id/eprint/189429
ISSN: 0098-8472
PURE UUID: ba4d3ba3-9c9f-4180-8c5f-3f8f1049fc3e

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Date deposited: 02 Jun 2011 08:46
Last modified: 14 Mar 2024 03:36

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Contributors

Author: Anna Barabasz
Author: Rebecca Fay Mills
Author: Ewa Trojanowska
Author: Danuta Maria Antosiewicz

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