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Expression of AtECA3 in tobacco modifies its responses to manganese, zinc and calcium

Barabasz, Anna, Mills, Rebecca Fay, Trojanowska, Ewa, Williams, Lorraine Elizabeth and Antosiewicz, Danuta Maria (2011) Expression of AtECA3 in tobacco modifies its responses to manganese, zinc and calcium Environmental and Experimental Botany, 72, (2), pp. 202-209.

Record type: Article

Abstract

Mn- and Zn-deficiency or excess reduce plant growth and development. Engineering plants with enhanced metal tolerance and accumulation is a major goal in phytoremediation/phytostabilization. Moreover, improved growth under unfavourable mineral conditions contributes to better crop production. In this study, ECA3 cDNA from Arabidopsis thaliana, encoding a P2A-ATPase, was introduced into Nicotiana tabacum var. Xanthi, to examine its value for modifying responses to cations, primarily Mn, Zn and Ca. AtECA3 was ectopically expressed under the CaMV 35S promoter. Transgenic and wild-type plants were tested under hydroponic conditions for their responses to a range of metal exposures (low, moderate and high concentrations), and their tolerance and accumulation were evaluated. AtECA3 expression resulted in better growth of plants at moderate levels of Mn (2 ?M) in the medium and enhanced tolerance to high Mn (100 ?M). Transgenic plants were also more tolerant to Ca-deficiency conditions, although they showed no differences to wild-type with respect to overall Ca levels. Transgene expression did not produce one unique pattern of Mn and Zn accumulation but instead depended on the external concentration of the particular metal supplied. Thus the enhancement of plant productivity at moderate Mn levels and increased Mn tolerance at high (toxic) Mn supply, as well as the slight increase in Ca-deficiency tolerance seen in ECA3-transformed plants indicates that this gene could be useful in plant biotechnological strategies.

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More information

Published date: September 2011
Keywords: ateca3, manganese, p2A-atpase, transformation, zinc, calcium

Identifiers

Local EPrints ID: 189429
URI: http://eprints.soton.ac.uk/id/eprint/189429
ISSN: 0098-8472
PURE UUID: ba4d3ba3-9c9f-4180-8c5f-3f8f1049fc3e

Catalogue record

Date deposited: 02 Jun 2011 08:46
Last modified: 26 Sep 2017 06:25

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Contributors

Author: Anna Barabasz
Author: Rebecca Fay Mills
Author: Ewa Trojanowska
Author: Danuta Maria Antosiewicz

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