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Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues

Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues
Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues
We have identified succinic semialdehyde dehydrogenase protein in rat and human neural and nonneural tissues. Tissue localization was determined by enzymatic assay and by western immunoblotting using polyclonal antibodies raised in rabbit against the purified rat brain protein. Although brain shows the highest level of succinic semialdehyde dehydrogenase activity, substantial amounts of enzyme activity occur in mammalian liver, pituitary, heart, and ovary. We further demonstrate the absence of succinic semialdehyde dehydrogenase enzyme activity and protein in brain, liver, and kidney tissue samples from an individual affected with succinic semialdehyde dehydrogenase deficiency, thereby verifying the specificity of our antibodies.
succinic semialdehyde dehydrogenase, neural tissue, nonneural tissue, spectrophotometry, immunoblot, inborn error of metabolism
0022-3042
851-855
Chambliss, Ken L.
8ba2481e-4138-465a-b991-fe2b279aa37c
Zhang, Yu-An
06311762-cae9-4a25-9d1a-c14e94a2b5a1
Rossier, Eva
38a8e088-e154-47d3-b443-04dd0a3cb206
Vollmer, Brigitte
044f8b55-ba36-4fb2-8e7e-756ab77653ba
Gibson, K. Michael
5dff37df-e4d3-4f09-a523-cfdbd4d642a0
Chambliss, Ken L.
8ba2481e-4138-465a-b991-fe2b279aa37c
Zhang, Yu-An
06311762-cae9-4a25-9d1a-c14e94a2b5a1
Rossier, Eva
38a8e088-e154-47d3-b443-04dd0a3cb206
Vollmer, Brigitte
044f8b55-ba36-4fb2-8e7e-756ab77653ba
Gibson, K. Michael
5dff37df-e4d3-4f09-a523-cfdbd4d642a0

Chambliss, Ken L., Zhang, Yu-An, Rossier, Eva, Vollmer, Brigitte and Gibson, K. Michael (1995) Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues. Journal of Neurochemistry, 65 (2), 851-855. (doi:10.1046/j.1471-4159.1995.65020851.x). (PMID:7616245)

Record type: Article

Abstract

We have identified succinic semialdehyde dehydrogenase protein in rat and human neural and nonneural tissues. Tissue localization was determined by enzymatic assay and by western immunoblotting using polyclonal antibodies raised in rabbit against the purified rat brain protein. Although brain shows the highest level of succinic semialdehyde dehydrogenase activity, substantial amounts of enzyme activity occur in mammalian liver, pituitary, heart, and ovary. We further demonstrate the absence of succinic semialdehyde dehydrogenase enzyme activity and protein in brain, liver, and kidney tissue samples from an individual affected with succinic semialdehyde dehydrogenase deficiency, thereby verifying the specificity of our antibodies.

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More information

Published date: August 1995
Keywords: succinic semialdehyde dehydrogenase, neural tissue, nonneural tissue, spectrophotometry, immunoblot, inborn error of metabolism

Identifiers

Local EPrints ID: 189531
URI: http://eprints.soton.ac.uk/id/eprint/189531
ISSN: 0022-3042
PURE UUID: 2fe6bef5-9892-4177-8c34-3fe3ec37c9c4
ORCID for Brigitte Vollmer: ORCID iD orcid.org/0000-0003-4088-5336

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Date deposited: 02 Jun 2011 15:02
Last modified: 07 Oct 2020 04:32

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