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Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues

Record type: Article

We have identified succinic semialdehyde dehydrogenase protein in rat and human neural and nonneural tissues. Tissue localization was determined by enzymatic assay and by western immunoblotting using polyclonal antibodies raised in rabbit against the purified rat brain protein. Although brain shows the highest level of succinic semialdehyde dehydrogenase activity, substantial amounts of enzyme activity occur in mammalian liver, pituitary, heart, and ovary. We further demonstrate the absence of succinic semialdehyde dehydrogenase enzyme activity and protein in brain, liver, and kidney tissue samples from an individual affected with succinic semialdehyde dehydrogenase deficiency, thereby verifying the specificity of our antibodies.

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Citation

Chambliss, Ken L., Zhang, Yu-An, Rossier, Eva and Gibson, K. Michael (1995) Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues Journal of Neurochemistry, 65, (2), pp. 851-855. (doi:10.1046/j.1471-4159.1995.65020851.x). (PMID:7616245).

More information

Published date: August 1995
Keywords: succinic semialdehyde dehydrogenase, neural tissue, nonneural tissue, spectrophotometry, immunoblot, inborn error of metabolism

Identifiers

Local EPrints ID: 189531
URI: http://eprints.soton.ac.uk/id/eprint/189531
ISSN: 0022-3042
PURE UUID: 2fe6bef5-9892-4177-8c34-3fe3ec37c9c4

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Date deposited: 02 Jun 2011 15:02
Last modified: 18 Jul 2017 11:40

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Contributors

Author: Ken L. Chambliss
Author: Yu-An Zhang
Author: Eva Rossier
Author: K. Michael Gibson

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