We have identified succinic semialdehyde dehydrogenase protein in rat and human neural and nonneural tissues. Tissue localization was determined by enzymatic assay and by western immunoblotting using polyclonal antibodies raised in rabbit against the purified rat brain protein. Although brain shows the highest level of succinic semialdehyde dehydrogenase activity, substantial amounts of enzyme activity occur in mammalian liver, pituitary, heart, and ovary. We further demonstrate the absence of succinic semialdehyde dehydrogenase enzyme activity and protein in brain, liver, and kidney tissue samples from an individual affected with succinic semialdehyde dehydrogenase deficiency, thereby verifying the specificity of our antibodies.
Full text not available from this repository.
Citation
Chambliss, Ken L., Zhang, Yu-An, Rossier, Eva and Gibson, K. Michael (1995) Enzymatic and immunologic identification of succinic semialdehyde dehydrogenase in rat and human neural and nonneural tissues Journal of Neurochemistry, 65, (2), pp. 851-855. (doi:10.1046/j.1471-4159.1995.65020851.x). (PMID:7616245).
More information
Identifiers
Catalogue record
Export record
Altmetrics
Contributors
University divisions
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.