The mechanism of DNA repair by uracil-DNA glycosylase: Studies using nucleotide analogues
The mechanism of DNA repair by uracil-DNA glycosylase: Studies using nucleotide analogues
2',4'-Dideoxy-4'-methyleneuridine incorporated into oligodeoxynucleotides forms regular B-DNA duplexes as shown by Tm and CD measurements. Such oligomers are not cleaved by the DNA repair enzyme, UDG, which cleaves the glycosylic bond in dU but not in dT nor in dC nucleosides in single stranded and double stranded DNA. Differential binding of oligomers containing carbadU, 4'-thiodU, and dU residues to wild type and mutant UDG proteins identify an essential role for the furanose 4'-oxygen in recognition and cleavage of dU residues in DNA.
escherichia-coli, cytosine residues, structural basis, excision, catalysis, cyclopentane, glycosidase, deamination, nucleosides, inhibitor
1505-1516
Rosler, A.
e4327d04-6f65-4b37-b092-91f88d9d3343
Panayotou, G.
29c965c5-1dc5-411d-9190-9caa94a2751c
Hornby, D. P.
51f07c26-3200-473d-be09-5bd1090c03c3
Barlow, T.
90765474-cd91-441e-ba07-be58059d0951
Brown, T.
65b220ab-5839-4e03-b923-97694339baaf
Pearl, L. H.
e203850d-9a44-4cf2-b500-0c67699a2202
Savva, R.
f57609b3-9670-4777-9292-206078c21b4d
Blackburn, G. M.
00288d7b-f8c2-420e-a125-370841f070ab
2000
Rosler, A.
e4327d04-6f65-4b37-b092-91f88d9d3343
Panayotou, G.
29c965c5-1dc5-411d-9190-9caa94a2751c
Hornby, D. P.
51f07c26-3200-473d-be09-5bd1090c03c3
Barlow, T.
90765474-cd91-441e-ba07-be58059d0951
Brown, T.
65b220ab-5839-4e03-b923-97694339baaf
Pearl, L. H.
e203850d-9a44-4cf2-b500-0c67699a2202
Savva, R.
f57609b3-9670-4777-9292-206078c21b4d
Blackburn, G. M.
00288d7b-f8c2-420e-a125-370841f070ab
Rosler, A., Panayotou, G., Hornby, D. P., Barlow, T., Brown, T., Pearl, L. H., Savva, R. and Blackburn, G. M.
(2000)
The mechanism of DNA repair by uracil-DNA glycosylase: Studies using nucleotide analogues.
Nucleosides Nucleotides and Nucleic Acids, 19 (10-12), .
Abstract
2',4'-Dideoxy-4'-methyleneuridine incorporated into oligodeoxynucleotides forms regular B-DNA duplexes as shown by Tm and CD measurements. Such oligomers are not cleaved by the DNA repair enzyme, UDG, which cleaves the glycosylic bond in dU but not in dT nor in dC nucleosides in single stranded and double stranded DNA. Differential binding of oligomers containing carbadU, 4'-thiodU, and dU residues to wild type and mutant UDG proteins identify an essential role for the furanose 4'-oxygen in recognition and cleavage of dU residues in DNA.
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Published date: 2000
Keywords:
escherichia-coli, cytosine residues, structural basis, excision, catalysis, cyclopentane, glycosidase, deamination, nucleosides, inhibitor
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Local EPrints ID: 18977
URI: http://eprints.soton.ac.uk/id/eprint/18977
PURE UUID: 0fe2dad2-3fff-46ad-a119-7404eab1725f
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Date deposited: 18 Jan 2006
Last modified: 08 Jan 2022 01:00
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Contributors
Author:
A. Rosler
Author:
G. Panayotou
Author:
D. P. Hornby
Author:
T. Barlow
Author:
T. Brown
Author:
L. H. Pearl
Author:
R. Savva
Author:
G. M. Blackburn
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