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Sustaining olfaction at low salinities: mapping ion flux associated with the olfactory sensilla of the blue crab Callinectes sapidus

Sustaining olfaction at low salinities: mapping ion flux associated with the olfactory sensilla of the blue crab Callinectes sapidus
Sustaining olfaction at low salinities: mapping ion flux associated with the olfactory sensilla of the blue crab Callinectes sapidus
To test the hypothesis of a diffusion-generated, ionic/osmotic microenvironment within the olfactory sensilla (aesthetascs), flux gradients of Ca(2+) and K(+) associated with the external surfaces of these sensilla were spatially mapped using self-referencing, ion-selective microelectrodes. Blue crabs (Callinectes sapidus) acclimated to low-salinity conditions (15% sea water and fresh water) showed a net efflux of ions from the aesthetascs. The region of maximum flux associated with each aesthetasc conformed to that predicted from structural data and corresponded to the permeable region of the cuticle separating the olfactory dendrites from the external environment. Estimates of net flux from the entire tuft of aesthetascs for both Ca(2+) and K(+) fell within the predicted range on the basis of comparisons with (22)Na(+) flux measured previously and assuming a passive diffusion model of ion movement from the hemolymph to the sensillar lymph and, ultimately, to the external environment. The maximum concentrations of these ions measured deep within the tuft are discussed in the light of a potential across the aesthetascs that may limit ion efflux at low salinities.
0022-0949
3145-3152
Gleeson, R.A.
33f33865-f4e6-40ca-a75e-b1108d8bab7f
Hammar, K.
a39cddad-b4b1-4019-854f-3eb196bb9d5c
Smith, P.J.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Gleeson, R.A.
33f33865-f4e6-40ca-a75e-b1108d8bab7f
Hammar, K.
a39cddad-b4b1-4019-854f-3eb196bb9d5c
Smith, P.J.
003de469-9420-4f12-8f0e-8e8d76d28d6c

Gleeson, R.A., Hammar, K. and Smith, P.J. (2000) Sustaining olfaction at low salinities: mapping ion flux associated with the olfactory sensilla of the blue crab Callinectes sapidus. Journal of Experimental Biology, 203 (20), 3145-3152. (PMID:11003825)

Record type: Article

Abstract

To test the hypothesis of a diffusion-generated, ionic/osmotic microenvironment within the olfactory sensilla (aesthetascs), flux gradients of Ca(2+) and K(+) associated with the external surfaces of these sensilla were spatially mapped using self-referencing, ion-selective microelectrodes. Blue crabs (Callinectes sapidus) acclimated to low-salinity conditions (15% sea water and fresh water) showed a net efflux of ions from the aesthetascs. The region of maximum flux associated with each aesthetasc conformed to that predicted from structural data and corresponded to the permeable region of the cuticle separating the olfactory dendrites from the external environment. Estimates of net flux from the entire tuft of aesthetascs for both Ca(2+) and K(+) fell within the predicted range on the basis of comparisons with (22)Na(+) flux measured previously and assuming a passive diffusion model of ion movement from the hemolymph to the sensillar lymph and, ultimately, to the external environment. The maximum concentrations of these ions measured deep within the tuft are discussed in the light of a potential across the aesthetascs that may limit ion efflux at low salinities.

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Published date: October 2000

Identifiers

Local EPrints ID: 190213
URI: http://eprints.soton.ac.uk/id/eprint/190213
ISSN: 0022-0949
PURE UUID: cebfd29f-541f-4837-b93a-e85ec1c08f8f
ORCID for P.J. Smith: ORCID iD orcid.org/0000-0003-4400-6853

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Date deposited: 17 Jun 2011 13:29
Last modified: 26 Nov 2019 01:39

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Contributors

Author: R.A. Gleeson
Author: K. Hammar
Author: P.J. Smith ORCID iD

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