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Cadmium inhibits vacuolar H(+)ATPase-mediated acidification in the rat epididymis

Cadmium inhibits vacuolar H(+)ATPase-mediated acidification in the rat epididymis
Cadmium inhibits vacuolar H(+)ATPase-mediated acidification in the rat epididymis
In rats, an acidic luminal pH maintains sperm quiescence during storage in the epididymis. We recently showed that vacuolar H(+)ATPase-rich cells in the epididymis and vas deferens are involved in the acidification of these segments. Treatment of rats with cadmium (Cd) leads to alkalinization of this fluid by an unknown mechanism. Because Cd may affect H(+)ATPase function, we examined 1) the in vivo effect of Cd poisoning on H(+)ATPase-rich cell morphology and on the abundance and distribution of the 31-kDa H(+)ATPase subunit in cells along the rat epididymis, and 2) the in vitro effect of Cd on H(+)ATPase activity and function in the isolated vas deferens. Immunofluorescence and immunoblotting data from rats treated with Cd for 14-15 days (2 mg Cd/kg body mass/day) showed that 1) H(+)ATPase-positive cells regressed to a prepubertal phenotype, and 2) H(+)ATPase was lost from the apical pole of the cell and was redistributed into an intracellular compartment. In experiments in vitro, Cd inhibited bafilomycin-sensitive ATPase activity in isolated total cell membranes and, as measured using a proton-selective extracellular microelectrode, inhibited proton secretion in isolated vas deferens. We conclude that alkalinization of the tubule fluid in the epididymis and vas deferens of Cd-treated rats may result from the loss of functional H(+)ATPase enzyme in the cell apical domain as well as from a direct inhibition of H(+)ATPase function by Cd.
599-606
Herak-Kramberger, Carol M.
4c96a153-0855-4df6-a6ab-016478ee79b9
Sabolić, Ivan
6ed3c6fc-519f-494c-ba9a-4684c47d1cc7
Blanusa, Maja
e312c4e1-6a2e-4232-a950-27926432fac5
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Brown, Dennis
6938f113-e019-4a94-b66a-ae244ae2e6d7
Breton, Sylvie
2e794185-ff02-4aef-a2b3-a390eb5552b5
Herak-Kramberger, Carol M.
4c96a153-0855-4df6-a6ab-016478ee79b9
Sabolić, Ivan
6ed3c6fc-519f-494c-ba9a-4684c47d1cc7
Blanusa, Maja
e312c4e1-6a2e-4232-a950-27926432fac5
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Brown, Dennis
6938f113-e019-4a94-b66a-ae244ae2e6d7
Breton, Sylvie
2e794185-ff02-4aef-a2b3-a390eb5552b5

Herak-Kramberger, Carol M., Sabolić, Ivan, Blanusa, Maja, Smith, Peter J.S., Brown, Dennis and Breton, Sylvie (2000) Cadmium inhibits vacuolar H(+)ATPase-mediated acidification in the rat epididymis. Biology of Reproduction, 63 (2), 599-606. (doi:10.1095/?biolreprod63.2.599). (PMID:10906070)

Record type: Article

Abstract

In rats, an acidic luminal pH maintains sperm quiescence during storage in the epididymis. We recently showed that vacuolar H(+)ATPase-rich cells in the epididymis and vas deferens are involved in the acidification of these segments. Treatment of rats with cadmium (Cd) leads to alkalinization of this fluid by an unknown mechanism. Because Cd may affect H(+)ATPase function, we examined 1) the in vivo effect of Cd poisoning on H(+)ATPase-rich cell morphology and on the abundance and distribution of the 31-kDa H(+)ATPase subunit in cells along the rat epididymis, and 2) the in vitro effect of Cd on H(+)ATPase activity and function in the isolated vas deferens. Immunofluorescence and immunoblotting data from rats treated with Cd for 14-15 days (2 mg Cd/kg body mass/day) showed that 1) H(+)ATPase-positive cells regressed to a prepubertal phenotype, and 2) H(+)ATPase was lost from the apical pole of the cell and was redistributed into an intracellular compartment. In experiments in vitro, Cd inhibited bafilomycin-sensitive ATPase activity in isolated total cell membranes and, as measured using a proton-selective extracellular microelectrode, inhibited proton secretion in isolated vas deferens. We conclude that alkalinization of the tubule fluid in the epididymis and vas deferens of Cd-treated rats may result from the loss of functional H(+)ATPase enzyme in the cell apical domain as well as from a direct inhibition of H(+)ATPase function by Cd.

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Published date: August 2000

Identifiers

Local EPrints ID: 190219
URI: https://eprints.soton.ac.uk/id/eprint/190219
PURE UUID: a053e371-292d-4c1d-97d3-a38dfd2bf4ef
ORCID for Peter J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853

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Date deposited: 15 Jun 2011 12:29
Last modified: 06 Jun 2018 12:31

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Author: Carol M. Herak-Kramberger
Author: Ivan Sabolić
Author: Maja Blanusa
Author: Dennis Brown
Author: Sylvie Breton

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