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Arrangement of radial actin bundles in the growth cone of Aplysia bag cell neurons shows the immediate past history of filopodial behavior

Arrangement of radial actin bundles in the growth cone of Aplysia bag cell neurons shows the immediate past history of filopodial behavior
Arrangement of radial actin bundles in the growth cone of Aplysia bag cell neurons shows the immediate past history of filopodial behavior
Filopodia that protrude forward from the lamellipodium, located at the leading edge of a neuronal growth cone, are needed to guide the extension of a nerve cell. At the core of each filopodium an actin bundle forms and grows into the lamellipodium. By using kymographs of time-lapse polarized light images we examined the relationship between the behavior of the filopodia, the actin bundles immediately proximal to the filopodia, and the shapes and composition of actin bundles in the whole lamellipodium. We find that the shapes of actin bundles, such as tilt, fork, and fused zones, originate at the leading edge and are surprisingly well preserved during retrograde transport of the actin cytoskeleton in the whole lamellipodium. The number of filaments that make up the radial actin bundles, as displayed by their birefringence retardation, also is preserved during retrograde flow over a distance of 4-8 microm from the leading edge into the lamellipodium. Thus, the disposition of the actin bundles in the lamellipodium frozen at any time point preserves and portrays a history of the past behavior of actin bundles proximal to the filopodia and the behavior of the filopodia themselves. These findings suggest that the arrangement of actin bundles in static image records, such as electron or fluorescence micrographs of fixed and stained specimens, can in fact reveal the sequence of the past history of filopodial behavior and the generation, density, fusion, etc. of the filaments in the actin bundles.
nerve cell growth, cell motility, actin filaments, polarized light microscopy
0027-8424
7928-7931
Katoh, K
d69fd3c4-eddb-4833-a37c-03f766c8c3db
Hammar, K
7f28b7ee-a9b6-4e34-a5de-a62b3a3a2575
Smith, P J
003de469-9420-4f12-8f0e-8e8d76d28d6c
Oldenbourg, R
7e5cdb18-b46c-428e-a433-1d90ac2e5f94
Katoh, K
d69fd3c4-eddb-4833-a37c-03f766c8c3db
Hammar, K
7f28b7ee-a9b6-4e34-a5de-a62b3a3a2575
Smith, P J
003de469-9420-4f12-8f0e-8e8d76d28d6c
Oldenbourg, R
7e5cdb18-b46c-428e-a433-1d90ac2e5f94

(1999) Arrangement of radial actin bundles in the growth cone of Aplysia bag cell neurons shows the immediate past history of filopodial behavior. Proceedings of the National Academy of Sciences, 96 (14), 7928-7931. (doi:10.1073/pnas.96.14.7928). (PMID:1608453)

Record type: Article

Abstract

Filopodia that protrude forward from the lamellipodium, located at the leading edge of a neuronal growth cone, are needed to guide the extension of a nerve cell. At the core of each filopodium an actin bundle forms and grows into the lamellipodium. By using kymographs of time-lapse polarized light images we examined the relationship between the behavior of the filopodia, the actin bundles immediately proximal to the filopodia, and the shapes and composition of actin bundles in the whole lamellipodium. We find that the shapes of actin bundles, such as tilt, fork, and fused zones, originate at the leading edge and are surprisingly well preserved during retrograde transport of the actin cytoskeleton in the whole lamellipodium. The number of filaments that make up the radial actin bundles, as displayed by their birefringence retardation, also is preserved during retrograde flow over a distance of 4-8 microm from the leading edge into the lamellipodium. Thus, the disposition of the actin bundles in the lamellipodium frozen at any time point preserves and portrays a history of the past behavior of actin bundles proximal to the filopodia and the behavior of the filopodia themselves. These findings suggest that the arrangement of actin bundles in static image records, such as electron or fluorescence micrographs of fixed and stained specimens, can in fact reveal the sequence of the past history of filopodial behavior and the generation, density, fusion, etc. of the filaments in the actin bundles.

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Published date: 6 July 1999
Keywords: nerve cell growth, cell motility, actin filaments, polarized light microscopy

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Local EPrints ID: 190243
URI: http://eprints.soton.ac.uk/id/eprint/190243
ISSN: 0027-8424
PURE UUID: 91e64bdc-07f2-4529-9734-8d305e8f5942
ORCID for P J Smith: ORCID iD orcid.org/0000-0003-4400-6853

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Date deposited: 17 Jun 2011 09:25
Last modified: 06 Jun 2018 12:31

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Author: K Katoh
Author: K Hammar
Author: P J Smith ORCID iD
Author: R Oldenbourg

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