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Noninvasive measurement of hydrogen and potassium ion flux from single cells and epithelial structures

Record type: Article

This review introduces new developments in a technique for measuring the movement of ions across the plasma membrane. With the use of a self-referencing ion-selective (Seris) probe, transport mechanisms can be studied on a variety of preparations ranging from tissues to single cells. In this paper we illustrate this versatility with examples from the vas deferens and inner ear epithelium to large and small single cells represented by mouse single-cell embryos and rat microglia. Potassium and hydrogen ion fluxes are studied and pharmacological manipulation of the signals are reported. The strengths of the self-referencing technique are reviewed with regard to biological applications, and the expansion of self-referencing probes to include electrochemical and enzyme-based sensors is discussed.

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Citation

Smith, P.J. and Trimarchi, J. (2001) Noninvasive measurement of hydrogen and potassium ion flux from single cells and epithelial structures American Journal of Physiology. Cell Physiology, 280, (1), C1-C11. (PMID:11121371).

More information

Published date: January 2001
Keywords: ion-selective electrodes, self-referencing probes, vas deferens, embryos, microglia, inner ear

Identifiers

Local EPrints ID: 190269
URI: http://eprints.soton.ac.uk/id/eprint/190269
ISSN: 0363-6143
PURE UUID: 18521e94-d8c3-4bbf-a808-eb19be0d40fb
ORCID for P.J. Smith: ORCID iD orcid.org/0000-0003-4400-6853

Catalogue record

Date deposited: 17 Jun 2011 12:37
Last modified: 18 Jul 2017 11:38

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Contributors

Author: P.J. Smith ORCID iD
Author: J. Trimarchi

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