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Release and elementary mechanisms of nitric oxide in hair cells

Lv, Ping, Rodriguez-Contreras, Adrian, Kim, Hyo Jeong, Zhu, Jun, Wei, Dongguang, Choong-Ryoul, Sihn, Eastwood, Emily, Mu, Karen, Levic, Snezana, Song, Haitao, Yevgeniy, Petrov Y, Smith, Peter J S and Yamoah, Ebenezer N (2010) Release and elementary mechanisms of nitric oxide in hair cells Journal of Neurophysiology, 103, (5), pp. 2494-505. (doi:10.1152/jn.00017.2010). (PMID:20220083).

Record type: Article


The enzyme nitric oxide (NO) synthase, that produces the signaling molecule NO, has been identified in several cell types in the inner ear. However, it is unclear whether a measurable quantity of NO is released in the inner ear to confer specific functions. Indeed, the functional significance of NO and the elementary cellular mechanism thereof are most uncertain. Here, we demonstrate that the sensory epithelia of the frog saccule release NO and explore its release mechanisms by using self-referencing NO-selective electrodes. Additionally, we investigated the functional effects of NO on electrical properties of hair cells and determined their underlying cellular mechanism. We show detectable amounts of NO are released by hair cells (>50 nM). Furthermore, a hair-cell efferent modulator acetylcholine produces at least a threefold increase in NO release. NO not only attenuated the baseline membrane oscillations but it also increased the magnitude of current required to generate the characteristic membrane potential oscillations. This resulted in a rightward shift in the frequency-current relationship and altered the excitability of hair cells. Our data suggest that these effects ensue because NO reduces whole cell Ca(2+) current and drastically decreases the open probability of single-channel events of the L-type and non L-type Ca(2+) channels in hair cells, an effect that is mediated through direct nitrosylation of the channel and activation of protein kinase G. Finally, NO increases the magnitude of Ca(2+)-activated K(+) currents via direct NO nitrosylation. We conclude that NO-mediated inhibition serves as a component of efferent nerve modulation of hair cells.

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Published date: May 2010
Organisations: Centre for Biological Sciences


Local EPrints ID: 190271
ISSN: 0022-3077
PURE UUID: 4ccbe684-bcd0-4943-85e1-71db5322c200
ORCID for Peter J S Smith: ORCID iD

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Date deposited: 13 Jun 2011 07:57
Last modified: 18 Jul 2017 11:38

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Author: Ping Lv
Author: Adrian Rodriguez-Contreras
Author: Hyo Jeong Kim
Author: Jun Zhu
Author: Dongguang Wei
Author: Sihn Choong-Ryoul
Author: Emily Eastwood
Author: Karen Mu
Author: Snezana Levic
Author: Haitao Song
Author: Petrov Y Yevgeniy
Author: Peter J S Smith ORCID iD
Author: Ebenezer N Yamoah

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