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Covalent attachment of osmium complexes to glucose oxidase and the application of the resulting modified enzyme in an enzyme switch responsive to glucose

Battaglini, F., Bartlett, P. N. and Wang, J. H. (2000) Covalent attachment of osmium complexes to glucose oxidase and the application of the resulting modified enzyme in an enzyme switch responsive to glucose Analytical Chemistry, 72, (3), pp. 502-509. (doi:10.1021/ac990321x).

Record type: Article

Abstract

Pyridine-based osmium complexes bearing either a carboxylate or aldehyde group were covalently attached to glucose oxidase and were shown to work as mediators for the reoxidation of the enzyme. For the complex containing the carboxylate group, the binding was made through carbodiimide coupling to the amine residues in the protein. For the complex containing the aldehyde group, the reductive coupling was carried out by condensation with the amino groups on the protein in the presence of sodium cyanoborohydride, Electrochemical studies show evidence for both intramolecular and intermolecular redox mediation for the electrochemical reoxidation of the modified glucose oxidases in the presence of glucose. The modified enzymes adsorbed on glassy carbon and platinum show different electrochemical responses for the two electrode materials, suggesting that orientation of the adsorbed enzyme is induced due to the interaction of the osmium complex with the different surfaces. Construction of enzyme switches based on these modified enzymes was carried out, and their responses were compared with those obtained using native glucose oxidase and a soluble redox mediator.

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More information

Published date: 1 February 2000
Keywords: direct electrical communication, chemically modified enzymes, cytochrome-c, metal-electrodes, direct electrochemistry, goldelectrode, redox reaction, fabrication, transistor, systems

Identifiers

Local EPrints ID: 19048
URI: http://eprints.soton.ac.uk/id/eprint/19048
ISSN: 0003-2700
PURE UUID: 59ce5b9a-3563-46fe-973c-496d7255b8dd
ORCID for P. N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

Catalogue record

Date deposited: 18 Jan 2006
Last modified: 17 Jul 2017 16:34

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Contributors

Author: F. Battaglini
Author: P. N. Bartlett ORCID iD
Author: J. H. Wang

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