Fast response, noninvasive, potentiometric microelectrodes resolve single potassium channel activity in the diffusive boundary layer of a single cell
Fast response, noninvasive, potentiometric microelectrodes resolve single potassium channel activity in the diffusive boundary layer of a single cell
Ion transport across the plasma membrane of cells involves an inevitable chemical modification of the solutions on both sides. Noninvasive, real-time detection of these subtle ionic signatures is most easily achieved in the extracellular diffusive boundary layer with electrochemical detection. In order to perform these measurements near single cells it is critical to use a device that enables high spatial and temporal resolution. While ion-selective microelectrodes (ISMs) provide the high spatial resolution, they give rise to orders of magnitude increase in the time constant and response time of the microsensors when compared to larger sensors. By constructing and using fast response ISMs biological events as brief as 10ms have been resolved. The signal-to-noise ratio is enhanced with self-referencing and signal processing techniques, enabling long-term monitoring of small magnitude, steady ion gradients and rapid ionic transients that reflect the physiological and metabolic activity of single cells
single channels, potentiometry, self-referencing, ion selective electrodes, potassium, membranes
1906-1913
Messerli, Mark A.
94a8bd34-95d7-4a52-b4d8-1ccded0e8fe7
Collis, Leon P.
66f825a3-f4a7-4e85-a1ca-ee16497b8a81
Smith, Peter J. S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
July 2009
Messerli, Mark A.
94a8bd34-95d7-4a52-b4d8-1ccded0e8fe7
Collis, Leon P.
66f825a3-f4a7-4e85-a1ca-ee16497b8a81
Smith, Peter J. S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Messerli, Mark A., Collis, Leon P. and Smith, Peter J. S.
(2009)
Fast response, noninvasive, potentiometric microelectrodes resolve single potassium channel activity in the diffusive boundary layer of a single cell.
Electroanalysis, 21 (17-18), .
(doi:10.1002/elan.200904618).
Abstract
Ion transport across the plasma membrane of cells involves an inevitable chemical modification of the solutions on both sides. Noninvasive, real-time detection of these subtle ionic signatures is most easily achieved in the extracellular diffusive boundary layer with electrochemical detection. In order to perform these measurements near single cells it is critical to use a device that enables high spatial and temporal resolution. While ion-selective microelectrodes (ISMs) provide the high spatial resolution, they give rise to orders of magnitude increase in the time constant and response time of the microsensors when compared to larger sensors. By constructing and using fast response ISMs biological events as brief as 10ms have been resolved. The signal-to-noise ratio is enhanced with self-referencing and signal processing techniques, enabling long-term monitoring of small magnitude, steady ion gradients and rapid ionic transients that reflect the physiological and metabolic activity of single cells
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Published date: July 2009
Keywords:
single channels, potentiometry, self-referencing, ion selective electrodes, potassium, membranes
Organisations:
University of Southampton
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Local EPrints ID: 190491
URI: http://eprints.soton.ac.uk/id/eprint/190491
ISSN: 1040-0397
PURE UUID: f9d6eb4b-d4c6-443d-b8fc-5a392d56ba44
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Date deposited: 13 Jun 2011 10:38
Last modified: 15 Mar 2024 03:38
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Author:
Mark A. Messerli
Author:
Leon P. Collis
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