Dielectrophoretic tweezer for isolating and manipulating target cells
Dielectrophoretic tweezer for isolating and manipulating target cells
The ability to isolate and accurately position single cells in three dimensions is becoming increasingly important in many areas of biological research. The authors describe the design, theoretical modelling and testing of a novel dielectrophoretic (DEP) tweezer for picking out and relocating single target cells. The device is constructed using facilities available in most electrophysiology laboratories, without the requirement of sophisticated and expensive microfabrication technology, and offers improved practical features over previously reported DEP tweezer designs. The DEP tweezer has been tested using transfected HEI-193 human schwannoma cells, with visual identification of the target cells being aided by labelling the incorporated gene product with a green fluorescent protein
1-7
Menachery, A.
141dcc97-491e-46fb-972b-a36dda1786d5
Graham, D.
47384ba8-2e3c-4892-84dd-2f3722844edc
Messerli, S.M.
6e70f53a-c8c7-41de-a00e-a2a86423a60b
Pethig, R.
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Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
March 2011
Menachery, A.
141dcc97-491e-46fb-972b-a36dda1786d5
Graham, D.
47384ba8-2e3c-4892-84dd-2f3722844edc
Messerli, S.M.
6e70f53a-c8c7-41de-a00e-a2a86423a60b
Pethig, R.
e58f40b8-85b0-4982-89a9-56d781eea024
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Menachery, A., Graham, D., Messerli, S.M., Pethig, R. and Smith, P.J.S.
(2011)
Dielectrophoretic tweezer for isolating and manipulating target cells.
IET Nanobiotechnology, 5 (1), .
(doi:10.1049/iet-nbt.2010.0010).
Abstract
The ability to isolate and accurately position single cells in three dimensions is becoming increasingly important in many areas of biological research. The authors describe the design, theoretical modelling and testing of a novel dielectrophoretic (DEP) tweezer for picking out and relocating single target cells. The device is constructed using facilities available in most electrophysiology laboratories, without the requirement of sophisticated and expensive microfabrication technology, and offers improved practical features over previously reported DEP tweezer designs. The DEP tweezer has been tested using transfected HEI-193 human schwannoma cells, with visual identification of the target cells being aided by labelling the incorporated gene product with a green fluorescent protein
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Published date: March 2011
Organisations:
University of Southampton
Identifiers
Local EPrints ID: 190495
URI: http://eprints.soton.ac.uk/id/eprint/190495
ISSN: 1751-8741
PURE UUID: 2aef6932-4bf2-442d-89ed-b93fe9544de3
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Date deposited: 13 Jun 2011 10:52
Last modified: 15 Mar 2024 03:38
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Author:
A. Menachery
Author:
D. Graham
Author:
S.M. Messerli
Author:
R. Pethig
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