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Dielectrophoretic tweezer for isolating and manipulating target cells

Dielectrophoretic tweezer for isolating and manipulating target cells
Dielectrophoretic tweezer for isolating and manipulating target cells
The ability to isolate and accurately position single cells in three dimensions is becoming increasingly important in many areas of biological research. The authors describe the design, theoretical modelling and testing of a novel dielectrophoretic (DEP) tweezer for picking out and relocating single target cells. The device is constructed using facilities available in most electrophysiology laboratories, without the requirement of sophisticated and expensive microfabrication technology, and offers improved practical features over previously reported DEP tweezer designs. The DEP tweezer has been tested using transfected HEI-193 human schwannoma cells, with visual identification of the target cells being aided by labelling the incorporated gene product with a green fluorescent protein
1751-8741
1-7
Menachery, A.
141dcc97-491e-46fb-972b-a36dda1786d5
Graham, D.
47384ba8-2e3c-4892-84dd-2f3722844edc
Messerli, S.M.
6e70f53a-c8c7-41de-a00e-a2a86423a60b
Pethig, R.
e58f40b8-85b0-4982-89a9-56d781eea024
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Menachery, A.
141dcc97-491e-46fb-972b-a36dda1786d5
Graham, D.
47384ba8-2e3c-4892-84dd-2f3722844edc
Messerli, S.M.
6e70f53a-c8c7-41de-a00e-a2a86423a60b
Pethig, R.
e58f40b8-85b0-4982-89a9-56d781eea024
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c

Menachery, A., Graham, D., Messerli, S.M., Pethig, R. and Smith, P.J.S. (2011) Dielectrophoretic tweezer for isolating and manipulating target cells. IET Nanobiotechnology, 5 (1), 1-7. (doi:10.1049/iet-nbt.2010.0010).

Record type: Article

Abstract

The ability to isolate and accurately position single cells in three dimensions is becoming increasingly important in many areas of biological research. The authors describe the design, theoretical modelling and testing of a novel dielectrophoretic (DEP) tweezer for picking out and relocating single target cells. The device is constructed using facilities available in most electrophysiology laboratories, without the requirement of sophisticated and expensive microfabrication technology, and offers improved practical features over previously reported DEP tweezer designs. The DEP tweezer has been tested using transfected HEI-193 human schwannoma cells, with visual identification of the target cells being aided by labelling the incorporated gene product with a green fluorescent protein

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Published date: March 2011
Organisations: University of Southampton

Identifiers

Local EPrints ID: 190495
URI: http://eprints.soton.ac.uk/id/eprint/190495
ISSN: 1751-8741
PURE UUID: 2aef6932-4bf2-442d-89ed-b93fe9544de3
ORCID for P.J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853

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Date deposited: 13 Jun 2011 10:52
Last modified: 29 Aug 2019 00:37

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Contributors

Author: A. Menachery
Author: D. Graham
Author: S.M. Messerli
Author: R. Pethig
Author: P.J.S. Smith ORCID iD

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