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Physiological and pharmacological characterizations of the larval Anopheles albimanus rectum support a change in protein distribution and/or function in varying salinities

Physiological and pharmacological characterizations of the larval Anopheles albimanus rectum support a change in protein distribution and/or function in varying salinities
Physiological and pharmacological characterizations of the larval Anopheles albimanus rectum support a change in protein distribution and/or function in varying salinities
Ion regulation is a biological process crucial to the survival of mosquito larvae and a major organ responsible for this regulation is the rectum. The recta of anopheline larvae are distinct from other subfamilies of mosquitoes in several ways, yet have not yet been characterized extensively. Here we characterize the two major cell types of the anopheline rectum, DAR and non-DAR cells, using histological, physiological, and pharmacological analyses. Proton flux was measured at the basal membrane of 2%- and 50%-artificial sea water-reared An. albimanus larvae using self-referencing ion-selective microelectrodes, and the two cell types were found to differ in basal membrane proton flux. Additionally, differences in the response of that flux to pharmacological inhibitors in larvae reared in 2% versus 50% ASW indicate changes in protein function between the two rearing conditions. Finally, histological analyses suggest that the non-DAR cells are structurally suited for mediating ion transport. These data support a model of rectal ion regulation in which the non-DAR cells have a resorptive function in freshwater-reared larvae and a secretive function in saline water-reared larvae. In this way, anopheline larvae may adapt to varying salinities
1095-6433
55-62
Smith, Kristin E.
81015c28-c365-430f-969f-9cae25bdcb73
Raymond, Steven L.
458c7346-ee40-429e-80ab-8ab1a4930468
Valenti, Micheala L.
b53551c6-cc42-4ab5-8fee-f105ae904aef
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Linser, Paul J.
07dff63e-2ba6-465d-9927-e1ae9b5bf210
Smith, Kristin E.
81015c28-c365-430f-969f-9cae25bdcb73
Raymond, Steven L.
458c7346-ee40-429e-80ab-8ab1a4930468
Valenti, Micheala L.
b53551c6-cc42-4ab5-8fee-f105ae904aef
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Linser, Paul J.
07dff63e-2ba6-465d-9927-e1ae9b5bf210

Smith, Kristin E., Raymond, Steven L., Valenti, Micheala L., Smith, Peter J.S. and Linser, Paul J. (2010) Physiological and pharmacological characterizations of the larval Anopheles albimanus rectum support a change in protein distribution and/or function in varying salinities. Comparative Biochemistry and Physiology. Part A: Molecular & Integrative Physiology, 157 (1), 55-62. (doi:10.1016/j.cbpa.2010.05.002). (PMID:20460167)

Record type: Article

Abstract

Ion regulation is a biological process crucial to the survival of mosquito larvae and a major organ responsible for this regulation is the rectum. The recta of anopheline larvae are distinct from other subfamilies of mosquitoes in several ways, yet have not yet been characterized extensively. Here we characterize the two major cell types of the anopheline rectum, DAR and non-DAR cells, using histological, physiological, and pharmacological analyses. Proton flux was measured at the basal membrane of 2%- and 50%-artificial sea water-reared An. albimanus larvae using self-referencing ion-selective microelectrodes, and the two cell types were found to differ in basal membrane proton flux. Additionally, differences in the response of that flux to pharmacological inhibitors in larvae reared in 2% versus 50% ASW indicate changes in protein function between the two rearing conditions. Finally, histological analyses suggest that the non-DAR cells are structurally suited for mediating ion transport. These data support a model of rectal ion regulation in which the non-DAR cells have a resorptive function in freshwater-reared larvae and a secretive function in saline water-reared larvae. In this way, anopheline larvae may adapt to varying salinities

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Published date: May 2010
Organisations: University of Southampton

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Local EPrints ID: 190497
URI: http://eprints.soton.ac.uk/id/eprint/190497
ISSN: 1095-6433
PURE UUID: 7fe71da4-ffb9-41e6-9be3-fd2dfe5e96a4
ORCID for Peter J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853

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Date deposited: 13 Jun 2011 11:55
Last modified: 20 Jul 2019 00:44

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Author: Kristin E. Smith
Author: Steven L. Raymond
Author: Micheala L. Valenti
Author: Paul J. Linser

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