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Stage-dependent effects of epidermal growth factor on Ca2+ efflux in mouse oocytes

Stage-dependent effects of epidermal growth factor on Ca2+ efflux in mouse oocytes
Stage-dependent effects of epidermal growth factor on Ca2+ efflux in mouse oocytes
Epidermal growth factor (EGF) has received much attention recently for its positive effects on mammalian oocyte maturation and embryo development and its potential importance in cytoplasmic maturation of oocytes. Calcium (Ca2+) homeostasis in germinal vesicle stage oocytes has also been suggested to play a role in cytoplasmic maturation. This study examined the effects of EGF on Ca2+ mobilization as measured by its efflux from mouse oocytes at three time periods throughout maturation (0–4 hr, 4–8 hr, and 12 hr). Immature cumulus oocyte complexes (COCs) removed from the ovary for less than 4 hr exhibit oscillations in Ca2+ efflux that initiated 5–30 min following EGF stimulation. This response was not observed in COCs matured for 4–8 hr or 12 hr or in unstimulated 0–4 hr COCs. Denuded oocytes and cumulus cells did not show the same response to EGF (8.2 nM and 16.4 nM). Immunohistochemistry for detection of the EGF receptor along with EGF internalization studies showed that receptors are present both on cumulus cells and the oocyte but EGF appears to be internalized mainly by the cumulus cells. These data demonstrate that EGF induces oscillations in Ca2+ efflux in COCs 0–4 hr old and this response is mediated by the cumulus cells. Mol. Reprod. Dev. 53:244–253, 1999.
egf, oocyte, mouse, maturation, calcium
1040-452X
244-253
Hill, J.L.
167b2096-513a-44d1-b42a-b15770eb7aac
Hammar, K.
a39cddad-b4b1-4019-854f-3eb196bb9d5c
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Gross, D.J.
be34918d-31bb-4daa-ae56-8e8bd5052601
Hill, J.L.
167b2096-513a-44d1-b42a-b15770eb7aac
Hammar, K.
a39cddad-b4b1-4019-854f-3eb196bb9d5c
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Gross, D.J.
be34918d-31bb-4daa-ae56-8e8bd5052601

Hill, J.L., Hammar, K., Smith, P.J.S. and Gross, D.J. (1999) Stage-dependent effects of epidermal growth factor on Ca2+ efflux in mouse oocytes. Molecular Reproduction and Development, 53 (2), 244-253. (doi:10.1002/(SICI)1098-2795(199906)53:2<244::AID-MRD13>3.0.CO;2-7). (PMID:10331462)

Record type: Article

Abstract

Epidermal growth factor (EGF) has received much attention recently for its positive effects on mammalian oocyte maturation and embryo development and its potential importance in cytoplasmic maturation of oocytes. Calcium (Ca2+) homeostasis in germinal vesicle stage oocytes has also been suggested to play a role in cytoplasmic maturation. This study examined the effects of EGF on Ca2+ mobilization as measured by its efflux from mouse oocytes at three time periods throughout maturation (0–4 hr, 4–8 hr, and 12 hr). Immature cumulus oocyte complexes (COCs) removed from the ovary for less than 4 hr exhibit oscillations in Ca2+ efflux that initiated 5–30 min following EGF stimulation. This response was not observed in COCs matured for 4–8 hr or 12 hr or in unstimulated 0–4 hr COCs. Denuded oocytes and cumulus cells did not show the same response to EGF (8.2 nM and 16.4 nM). Immunohistochemistry for detection of the EGF receptor along with EGF internalization studies showed that receptors are present both on cumulus cells and the oocyte but EGF appears to be internalized mainly by the cumulus cells. These data demonstrate that EGF induces oscillations in Ca2+ efflux in COCs 0–4 hr old and this response is mediated by the cumulus cells. Mol. Reprod. Dev. 53:244–253, 1999.

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Published date: June 1999
Keywords: egf, oocyte, mouse, maturation, calcium

Identifiers

Local EPrints ID: 190521
URI: http://eprints.soton.ac.uk/id/eprint/190521
ISSN: 1040-452X
PURE UUID: 9d225838-73a2-471f-aa96-0ada6ee21193
ORCID for P.J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853

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Date deposited: 20 Jun 2011 09:07
Last modified: 26 Nov 2019 01:39

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Author: J.L. Hill
Author: K. Hammar
Author: P.J.S. Smith ORCID iD
Author: D.J. Gross

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