Multitip scanning bio-Kelvin probe
Multitip scanning bio-Kelvin probe
We have developed a novel multitip scanning Kelvin probe which can measure changes in biological surface potential ΔVs to within 2 mV and, quasi-simultaneously monitor displacement to <1 µm. The control and measurement subcomponents are PC based and incorporate a flexible user interface permitting software control of each individual tip, measurement, and scan parameters. We review the mode of operation and design features of the scanning bio-Kelvin probe including tip steering, signal processing, tip calibration, and novel tip tracking/dithering routines. This system uniquely offers both tip-to-sample spacing control (which is essential to avoid spurious changes in ΔVs due to variations in mean spacing) and a dithering routine to maintain tip orientation to the biological specimen, irrespective of the latter’s movement. These features permit long term (>48 h) “active” tracking of the displacement and biopotentials developed along and around a plant shoot in response to an environmental stimulus, e.g., differential illumination (phototropism) or changes in orientation (gravitropism).
1842
Baikie, I.D.
0570b8fd-f83f-48eb-bfa5-0fd06f9aa56a
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Porterfield, D.M.
90790fa2-b4d8-4d76-94b6-cb50591c1c2a
Estrup, P.J.
08c01710-73ac-4c7f-bfd9-6cf1e37da98d
March 1999
Baikie, I.D.
0570b8fd-f83f-48eb-bfa5-0fd06f9aa56a
Smith, P.J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Porterfield, D.M.
90790fa2-b4d8-4d76-94b6-cb50591c1c2a
Estrup, P.J.
08c01710-73ac-4c7f-bfd9-6cf1e37da98d
Baikie, I.D., Smith, P.J.S., Porterfield, D.M. and Estrup, P.J.
(1999)
Multitip scanning bio-Kelvin probe.
Review of Scientific Instruments, 70 (3), .
(doi:10.1063/1.1149678).
Abstract
We have developed a novel multitip scanning Kelvin probe which can measure changes in biological surface potential ΔVs to within 2 mV and, quasi-simultaneously monitor displacement to <1 µm. The control and measurement subcomponents are PC based and incorporate a flexible user interface permitting software control of each individual tip, measurement, and scan parameters. We review the mode of operation and design features of the scanning bio-Kelvin probe including tip steering, signal processing, tip calibration, and novel tip tracking/dithering routines. This system uniquely offers both tip-to-sample spacing control (which is essential to avoid spurious changes in ΔVs due to variations in mean spacing) and a dithering routine to maintain tip orientation to the biological specimen, irrespective of the latter’s movement. These features permit long term (>48 h) “active” tracking of the displacement and biopotentials developed along and around a plant shoot in response to an environmental stimulus, e.g., differential illumination (phototropism) or changes in orientation (gravitropism).
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Published date: March 1999
Identifiers
Local EPrints ID: 190523
URI: http://eprints.soton.ac.uk/id/eprint/190523
ISSN: 0034-6748
PURE UUID: 430eadc3-2fb7-47ff-bef4-53233b359feb
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Date deposited: 20 Jun 2011 09:21
Last modified: 15 Mar 2024 03:38
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Author:
I.D. Baikie
Author:
D.M. Porterfield
Author:
P.J. Estrup
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