Long-term culture of fully differentiated adult insect neurons
Long-term culture of fully differentiated adult insect neurons
An identified group of cells, dorsal unpaired median (DUM) neurons, isolated from the central nervous system of adult cockroaches can be grown in vitro for extended periods. These cells often develop morphological characteristics that differ from their in situ appearance but physiological experiments demonstrate that they retain their distinctive in vivo membrane properties. A method for culturing insect CNS neurons, which includes the use of a haemolymph-derived growth-enhancing factor is described, and this technique is compared with other attempts to develop an efficient system for producing in vitro preparations of well-defined, identifiable neurons that would provide a model system for aspects of neuron developmental growth, repair and function.
periplaneta americana, cockroach dum neuron, growth factor, insect hemolymph
113-122
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Howes, Elizabeth A.
efe4947b-1f7d-4de8-b8a6-7eebdf9754a3
21 October 1996
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Howes, Elizabeth A.
efe4947b-1f7d-4de8-b8a6-7eebdf9754a3
Smith, Peter J.S. and Howes, Elizabeth A.
(1996)
Long-term culture of fully differentiated adult insect neurons.
Journal of Neuroscience Methods, 69 (1), .
(doi:10.1016/S0165-0270(96)00026-X).
Abstract
An identified group of cells, dorsal unpaired median (DUM) neurons, isolated from the central nervous system of adult cockroaches can be grown in vitro for extended periods. These cells often develop morphological characteristics that differ from their in situ appearance but physiological experiments demonstrate that they retain their distinctive in vivo membrane properties. A method for culturing insect CNS neurons, which includes the use of a haemolymph-derived growth-enhancing factor is described, and this technique is compared with other attempts to develop an efficient system for producing in vitro preparations of well-defined, identifiable neurons that would provide a model system for aspects of neuron developmental growth, repair and function.
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Published date: 21 October 1996
Keywords:
periplaneta americana, cockroach dum neuron, growth factor, insect hemolymph
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Local EPrints ID: 190543
URI: http://eprints.soton.ac.uk/id/eprint/190543
ISSN: 0165-0270
PURE UUID: 67492441-a4fc-4696-93c4-8d9a3bdef018
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Date deposited: 15 Jun 2011 12:17
Last modified: 15 Mar 2024 03:39
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Author:
Elizabeth A. Howes
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