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Proteins and lipids define the diffusional field of nitric oxide

Proteins and lipids define the diffusional field of nitric oxide
Proteins and lipids define the diffusional field of nitric oxide
Nitric oxide (NO) fluxes released from the surface of individual activated macrophages or cells localized in small aggregates were measured with a novel polarographic self-referencing microsensor. NO fluxes could be detected at distances from the cells of 100–500 ?m. The initial flux and the distance from the cells at which NO could be detected were directly related to the number of cells in the immediate vicinity of the probe releasing NO. Thus, whereas NO fluxes of ?1 pmol · cm?2 · s?1were measured from individual macrophages, aggregates composed of groups of cells varying in number from 18 to 48 cells produced NO fluxes of between ?4 and 10 pmol · cm?2 · s?1. NO fluxes required the presence of l-arginine. Signals were significantly reduced by the addition of hemoglobin and byN-nitro-l-arginine methyl ester. NO fluxes were greatest when the sensor was placed immediately adjacent to cell membranes and declined as the distance from the cell increased. The NO signal was markedly reduced in the presence of the protein albumin but not by either oxidized or reduced glutathione. A reduction in the NO signal was also noted after the addition of lipid micelles to the culture medium. These results demonstrate that NO can be detected at significant distances from the cell of origin. In addition, both proteins and lipids strongly influence the net movement of free NO from macrophages. This suggests that these tissue components play an important role in regulating the biological activity of NO.

nitric oxide flux, nitric oxide synthase, self-referencing electrode
1040-0605
L904-L912
Porterfield, D. Marshall
ad367ffb-cd69-4c9e-ac0c-f7f048538518
Laskin, Jeffrey D.
8c4377b4-ba21-4811-8cb1-35904f35e1d5
Jung, Sung-Kwon
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Malchow, Robert Paul
b0b1afa6-0f50-488c-9028-7f05d1932ee1
Billack, Blase
ee08b534-cf8d-4d8d-a4ed-686d094ef52f
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Heck, Diane E.
03a96ab5-efa2-4374-a29f-a9ec70101fc0
Porterfield, D. Marshall
ad367ffb-cd69-4c9e-ac0c-f7f048538518
Laskin, Jeffrey D.
8c4377b4-ba21-4811-8cb1-35904f35e1d5
Jung, Sung-Kwon
9b10457b-933f-4247-a75a-62320f744dc7
Malchow, Robert Paul
b0b1afa6-0f50-488c-9028-7f05d1932ee1
Billack, Blase
ee08b534-cf8d-4d8d-a4ed-686d094ef52f
Smith, Peter J.S.
003de469-9420-4f12-8f0e-8e8d76d28d6c
Heck, Diane E.
03a96ab5-efa2-4374-a29f-a9ec70101fc0

Porterfield, D. Marshall, Laskin, Jeffrey D., Jung, Sung-Kwon, Malchow, Robert Paul, Billack, Blase, Smith, Peter J.S. and Heck, Diane E. (2001) Proteins and lipids define the diffusional field of nitric oxide. American Journal of Physiology: Lung Cellular and Molecular Physiology, 281 (4), L904-L912.

Record type: Article

Abstract

Nitric oxide (NO) fluxes released from the surface of individual activated macrophages or cells localized in small aggregates were measured with a novel polarographic self-referencing microsensor. NO fluxes could be detected at distances from the cells of 100–500 ?m. The initial flux and the distance from the cells at which NO could be detected were directly related to the number of cells in the immediate vicinity of the probe releasing NO. Thus, whereas NO fluxes of ?1 pmol · cm?2 · s?1were measured from individual macrophages, aggregates composed of groups of cells varying in number from 18 to 48 cells produced NO fluxes of between ?4 and 10 pmol · cm?2 · s?1. NO fluxes required the presence of l-arginine. Signals were significantly reduced by the addition of hemoglobin and byN-nitro-l-arginine methyl ester. NO fluxes were greatest when the sensor was placed immediately adjacent to cell membranes and declined as the distance from the cell increased. The NO signal was markedly reduced in the presence of the protein albumin but not by either oxidized or reduced glutathione. A reduction in the NO signal was also noted after the addition of lipid micelles to the culture medium. These results demonstrate that NO can be detected at significant distances from the cell of origin. In addition, both proteins and lipids strongly influence the net movement of free NO from macrophages. This suggests that these tissue components play an important role in regulating the biological activity of NO.

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More information

Published date: October 2001
Keywords: nitric oxide flux, nitric oxide synthase, self-referencing electrode

Identifiers

Local EPrints ID: 190601
URI: http://eprints.soton.ac.uk/id/eprint/190601
ISSN: 1040-0605
PURE UUID: 0bf9d60a-6753-418a-9b38-2bb805dd277b
ORCID for Peter J.S. Smith: ORCID iD orcid.org/0000-0003-4400-6853

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Date deposited: 17 Jun 2011 12:41
Last modified: 21 Nov 2021 03:04

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Contributors

Author: D. Marshall Porterfield
Author: Jeffrey D. Laskin
Author: Sung-Kwon Jung
Author: Robert Paul Malchow
Author: Blase Billack
Author: Diane E. Heck

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