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Tumor necrosis factor alpha (TNF-α) autoregulates its expression and induces adhesion molecule expression in asthma

Tumor necrosis factor alpha (TNF-α) autoregulates its expression and induces adhesion molecule expression in asthma
Tumor necrosis factor alpha (TNF-α) autoregulates its expression and induces adhesion molecule expression in asthma
Subjects with mild asthma underwent repeated low-dose allergen exposure and bronchial biopsies were examined for the expression of TNF-α and adhesion molecules. Bronchial biopsies from moderately severe asthmatics were then tested in an explant culture system to assess the effect of Der p and CDP-870, a TNF-α blocking pegylated-antibody Fab, on expression of TNF-α and adhesion molecules. Low-dose allergen challenge significantly upregulated sub-mucosal mast cells, TNF-α+ cells, and VCAM. When bronchial explants were exposed to Der p and CDP 870 for 24 h, CDP 870 caused a significant reduction in TNF-α release both at baseline and following stimulation with Der p allergen. The bronchial biopsies showed significant upregulation of TNF-α positive cells and ICAM-1 following exposure to Der p (p = 0.03) and this was reduced in the presence of CDP-870. So, allergen exposure up-regulates TNF-α expression in asthma and down-stream targets, including adhesion molecules that contribute to airway inflammation.
asthma, airway inflammation, tumor necrosis factor, adhesion molecule, allergen
1521-6616
18-25
Babu, Suresh K.
6dabb88a-b49d-4d9e-98ff-fb6ade561568
Puddicombe, Sarah M.
124e2c4e-ab9a-46f3-855c-b54ed0b61cc4
Arshad, Hasan S.
917e246d-2e60-472f-8d30-94b01ef28958
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Ward, Jonathan
a8107b98-069d-4263-bd70-d29d79f07edc
Gozzard, Neil
d6dccab8-d170-4280-9289-82acabebc156
Higgs, Gerry
dd509a4a-4162-4579-99ff-fbe2ffc31ba2
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Babu, Suresh K.
6dabb88a-b49d-4d9e-98ff-fb6ade561568
Puddicombe, Sarah M.
124e2c4e-ab9a-46f3-855c-b54ed0b61cc4
Arshad, Hasan S.
917e246d-2e60-472f-8d30-94b01ef28958
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Ward, Jonathan
a8107b98-069d-4263-bd70-d29d79f07edc
Gozzard, Neil
d6dccab8-d170-4280-9289-82acabebc156
Higgs, Gerry
dd509a4a-4162-4579-99ff-fbe2ffc31ba2
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38

Babu, Suresh K., Puddicombe, Sarah M., Arshad, Hasan S., Wilson, Susan J., Ward, Jonathan, Gozzard, Neil, Higgs, Gerry, Holgate, Stephen T. and Davies, Donna E. (2011) Tumor necrosis factor alpha (TNF-α) autoregulates its expression and induces adhesion molecule expression in asthma. Clinical Immunology, 140 (1), 18-25. (doi:10.1016/j.clim.2011.03.005). (PMID:21459047)

Record type: Article

Abstract

Subjects with mild asthma underwent repeated low-dose allergen exposure and bronchial biopsies were examined for the expression of TNF-α and adhesion molecules. Bronchial biopsies from moderately severe asthmatics were then tested in an explant culture system to assess the effect of Der p and CDP-870, a TNF-α blocking pegylated-antibody Fab, on expression of TNF-α and adhesion molecules. Low-dose allergen challenge significantly upregulated sub-mucosal mast cells, TNF-α+ cells, and VCAM. When bronchial explants were exposed to Der p and CDP 870 for 24 h, CDP 870 caused a significant reduction in TNF-α release both at baseline and following stimulation with Der p allergen. The bronchial biopsies showed significant upregulation of TNF-α positive cells and ICAM-1 following exposure to Der p (p = 0.03) and this was reduced in the presence of CDP-870. So, allergen exposure up-regulates TNF-α expression in asthma and down-stream targets, including adhesion molecules that contribute to airway inflammation.

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More information

Published date: July 2011
Keywords: asthma, airway inflammation, tumor necrosis factor, adhesion molecule, allergen

Identifiers

Local EPrints ID: 190891
URI: https://eprints.soton.ac.uk/id/eprint/190891
ISSN: 1521-6616
PURE UUID: bb082502-0710-40a3-a488-cb676ae3508a

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Date deposited: 15 Jun 2011 14:02
Last modified: 28 Jun 2018 16:31

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Contributors

Author: Suresh K. Babu
Author: Sarah M. Puddicombe
Author: Hasan S. Arshad
Author: Susan J. Wilson
Author: Jonathan Ward
Author: Neil Gozzard
Author: Gerry Higgs
Author: Donna E. Davies

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