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Diesel exhaust exposure enhances the expression of IL-13 in the bronchial epithelium of healthy subjects

Diesel exhaust exposure enhances the expression of IL-13 in the bronchial epithelium of healthy subjects
Diesel exhaust exposure enhances the expression of IL-13 in the bronchial epithelium of healthy subjects
Epidemiological studies have demonstrated adverse health effects of environmental pollution. Diesel exhaust (DE) is an important contributor to ambient particulate matter pollution. DE exposure has been shown to induce a pronounced inflammatory response in the airways, with an enhanced epithelial expression of IL-8, and Gro-alpha in healthy subjects. The present investigation was aimed to further characterise the epithelial response to DE in vivo, with particular reference to possible TH2 response, in non-atopic healthy subjects. To determine this response, 15 healthy, non-atopic non-smoking subjects with normal lung function were exposed to DE (PM10 300 microg/m3) and filtered air during 1 h on two separate randomised occasions. Bronchoscopy sampling of bronchial mucosal biopsies was performed 6 h after exposure. Immunohistochemical staining were performed using mAb for IL-10, IL-13 and IL-18 expression. DE exposure induced a significant increase in the expression of IL-13 in the bronchial epithelium cells, 2.1 (1.35-4.88) Md (Q1-Q3) vs. air 0.94 (0.53-1.23); P = 0.009. No significant changes were seen in IL-10 and IL-18 expression. This finding suggests an TH2-inflammatory response in the airways of non-atopic healthy individuals.
821-825
Pourazar, Jamshid
d41db41e-cb53-4399-8c77-aaed8ff153a6
Frew, Anthony J.
4887b766-67c6-4d69-940d-4c06c0890b76
Blomberg, Anders
b19c0008-dc79-425d-a05b-2fda60f020cf
Helleday, Ragnberth
26fa403b-f9f0-41fd-9332-78bc493b78e5
Kelly, Frank J.
8eda554f-c23c-4321-b5e2-b99a72dfd0aa
Wilson, Susan
21c6875d-6870-441b-ae7a-603562a646b8
Sandström, Thomas
77a4c11d-a84f-4a52-9bfe-4ec83f62363a
Pourazar, Jamshid
d41db41e-cb53-4399-8c77-aaed8ff153a6
Frew, Anthony J.
4887b766-67c6-4d69-940d-4c06c0890b76
Blomberg, Anders
b19c0008-dc79-425d-a05b-2fda60f020cf
Helleday, Ragnberth
26fa403b-f9f0-41fd-9332-78bc493b78e5
Kelly, Frank J.
8eda554f-c23c-4321-b5e2-b99a72dfd0aa
Wilson, Susan
21c6875d-6870-441b-ae7a-603562a646b8
Sandström, Thomas
77a4c11d-a84f-4a52-9bfe-4ec83f62363a

Pourazar, Jamshid, Frew, Anthony J., Blomberg, Anders, Helleday, Ragnberth, Kelly, Frank J., Wilson, Susan and Sandström, Thomas (2004) Diesel exhaust exposure enhances the expression of IL-13 in the bronchial epithelium of healthy subjects. Respiratory Medicine, 98 (9), 821-825. (doi:10.1016/j.rmed.2004.02.025). (PMID:15338792)

Record type: Article

Abstract

Epidemiological studies have demonstrated adverse health effects of environmental pollution. Diesel exhaust (DE) is an important contributor to ambient particulate matter pollution. DE exposure has been shown to induce a pronounced inflammatory response in the airways, with an enhanced epithelial expression of IL-8, and Gro-alpha in healthy subjects. The present investigation was aimed to further characterise the epithelial response to DE in vivo, with particular reference to possible TH2 response, in non-atopic healthy subjects. To determine this response, 15 healthy, non-atopic non-smoking subjects with normal lung function were exposed to DE (PM10 300 microg/m3) and filtered air during 1 h on two separate randomised occasions. Bronchoscopy sampling of bronchial mucosal biopsies was performed 6 h after exposure. Immunohistochemical staining were performed using mAb for IL-10, IL-13 and IL-18 expression. DE exposure induced a significant increase in the expression of IL-13 in the bronchial epithelium cells, 2.1 (1.35-4.88) Md (Q1-Q3) vs. air 0.94 (0.53-1.23); P = 0.009. No significant changes were seen in IL-10 and IL-18 expression. This finding suggests an TH2-inflammatory response in the airways of non-atopic healthy individuals.

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Published date: September 2004

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Local EPrints ID: 190919
URI: https://eprints.soton.ac.uk/id/eprint/190919
PURE UUID: a508e244-d85a-4218-a7a6-82bb7631f115

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Date deposited: 17 Jun 2011 08:57
Last modified: 20 Jul 2018 16:31

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Contributors

Author: Jamshid Pourazar
Author: Anthony J. Frew
Author: Anders Blomberg
Author: Ragnberth Helleday
Author: Frank J. Kelly
Author: Susan Wilson
Author: Thomas Sandström

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