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Acute exposure to diesel exhaust increases IL-8 and GRO-alpha production in healthy human airways

Acute exposure to diesel exhaust increases IL-8 and GRO-alpha production in healthy human airways
Acute exposure to diesel exhaust increases IL-8 and GRO-alpha production in healthy human airways
We have previously demonstrated that short-term exposure to diesel exhaust (DE) for 1 h induced a marked leukocytic infiltration in the airways of healthy human volunteers involving neutrophils, lymphocytes, and mast cells along with increases in several inflammatory mediators. We hypothesized that the leukocyte infiltration and the various inflammatory responses induced by DE were mediated by enhanced chemokine and cytokine production by resident cells of the airway tissue and lumen. To investigate this, 15 healthy human volunteers were exposed to diluted DE and air on two separate occasions for 1 h each in an exposure chamber. Fiberoptic bronchoscopy was performed 6 h after each exposure to obtain endobronchial biopsies and bronchial wash (BW) cells. Using reverse transcriptase/polymerase chain reaction enzyme-linked immunosorbent assay (RT-PCR ELISA), a novel and sensitive technique to quantify relative amounts of cytokine mRNA gene transcripts, and immunohistochemical staining with computer-assisted image analysis to quantify expression of cytokine protein in the bronchial tissue, we have demonstrated that DE enhanced gene transcription of interleukin-8 (IL-8) in the bronchial tissue and BW cells along with increases in IL-8 and growth-regulated oncogene-alpha (GRO-alpha) protein expression in the bronchial epithelium, and an accompanying trend toward an increase in IL-5 mRNA gene transcripts in the bronchial tissue. There were no significant changes in the gene transcript levels of interleukin-1B (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), interferon gamma (IFN-gamma), and granulocyte macrophage colony-stimulating factor (GM-CSF) either in the bronchial tissue or BW cells after DE exposure at this time point. These observations suggest an underlying mechanism for DE-induced airway leukocyte infiltration and offer a possible explanation for the association observed between ambient levels of particulate matter and various respiratory health outcome indices noted in epidemiological studies.
1073-449X
550-557
Salvi, Sundeep S.
15ae94c5-5ac0-4036-b4ed-0498026bd87b
Nordenhall, Charlotta
50d994bb-d4af-4b9c-a7d8-857009397433
Blomberg, Anders
b19c0008-dc79-425d-a05b-2fda60f020cf
Rudell, Bertil
654e08e8-0438-43b1-9e8d-6f3b8fc74c99
Pourazar, Jamshid
d41db41e-cb53-4399-8c77-aaed8ff153a6
Kelly, Frank J.
8eda554f-c23c-4321-b5e2-b99a72dfd0aa
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Sandström, Thomas
77a4c11d-a84f-4a52-9bfe-4ec83f62363a
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Frew, Anthony J.
4887b766-67c6-4d69-940d-4c06c0890b76
Salvi, Sundeep S.
15ae94c5-5ac0-4036-b4ed-0498026bd87b
Nordenhall, Charlotta
50d994bb-d4af-4b9c-a7d8-857009397433
Blomberg, Anders
b19c0008-dc79-425d-a05b-2fda60f020cf
Rudell, Bertil
654e08e8-0438-43b1-9e8d-6f3b8fc74c99
Pourazar, Jamshid
d41db41e-cb53-4399-8c77-aaed8ff153a6
Kelly, Frank J.
8eda554f-c23c-4321-b5e2-b99a72dfd0aa
Wilson, Susan J.
21c6875d-6870-441b-ae7a-603562a646b8
Sandström, Thomas
77a4c11d-a84f-4a52-9bfe-4ec83f62363a
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Frew, Anthony J.
4887b766-67c6-4d69-940d-4c06c0890b76

Salvi, Sundeep S., Nordenhall, Charlotta, Blomberg, Anders, Rudell, Bertil, Pourazar, Jamshid, Kelly, Frank J., Wilson, Susan J., Sandström, Thomas, Holgate, Stephen T. and Frew, Anthony J. (2000) Acute exposure to diesel exhaust increases IL-8 and GRO-alpha production in healthy human airways. American Journal of Respiratory and Critical Care Medicine, 161 (2), part 1, 550-557. (doi:10.1164/ajrccm.161.2.9905052). (PMID:10673199)

Record type: Article

Abstract

We have previously demonstrated that short-term exposure to diesel exhaust (DE) for 1 h induced a marked leukocytic infiltration in the airways of healthy human volunteers involving neutrophils, lymphocytes, and mast cells along with increases in several inflammatory mediators. We hypothesized that the leukocyte infiltration and the various inflammatory responses induced by DE were mediated by enhanced chemokine and cytokine production by resident cells of the airway tissue and lumen. To investigate this, 15 healthy human volunteers were exposed to diluted DE and air on two separate occasions for 1 h each in an exposure chamber. Fiberoptic bronchoscopy was performed 6 h after each exposure to obtain endobronchial biopsies and bronchial wash (BW) cells. Using reverse transcriptase/polymerase chain reaction enzyme-linked immunosorbent assay (RT-PCR ELISA), a novel and sensitive technique to quantify relative amounts of cytokine mRNA gene transcripts, and immunohistochemical staining with computer-assisted image analysis to quantify expression of cytokine protein in the bronchial tissue, we have demonstrated that DE enhanced gene transcription of interleukin-8 (IL-8) in the bronchial tissue and BW cells along with increases in IL-8 and growth-regulated oncogene-alpha (GRO-alpha) protein expression in the bronchial epithelium, and an accompanying trend toward an increase in IL-5 mRNA gene transcripts in the bronchial tissue. There were no significant changes in the gene transcript levels of interleukin-1B (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), interferon gamma (IFN-gamma), and granulocyte macrophage colony-stimulating factor (GM-CSF) either in the bronchial tissue or BW cells after DE exposure at this time point. These observations suggest an underlying mechanism for DE-induced airway leukocyte infiltration and offer a possible explanation for the association observed between ambient levels of particulate matter and various respiratory health outcome indices noted in epidemiological studies.

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Published date: February 2000

Identifiers

Local EPrints ID: 190939
URI: https://eprints.soton.ac.uk/id/eprint/190939
ISSN: 1073-449X
PURE UUID: 1c86d472-edc1-464d-8272-10d72bdd5fac

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Date deposited: 17 Jun 2011 08:18
Last modified: 20 Jul 2018 16:31

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