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Effect of inhaled platelet-activating factor on bronchial inflammation in atopic non-asthmatic subjects

Effect of inhaled platelet-activating factor on bronchial inflammation in atopic non-asthmatic subjects
Effect of inhaled platelet-activating factor on bronchial inflammation in atopic non-asthmatic subjects
We have investigated the effect of inhaled platelet-activating factor (PAF) on bronchial mucosal inflammation in 6 atopic non-asthmatic subjects in a double-blind, placebo-controlled, randomised and crossover study. On 2 study periods at least 4 weeks apart, fiberoptic bronchoscopy was performed 24 h after inhalation of either 200 micrograms PAF or methacholine (control) to obtain endobronchial biopsies. Immunocytochemistry using antibodies for trypase (AA1) and eosinophil cationic protein (EG2) was performed to enumerate mast cells and eosinophils, respectively, in the bronchial submucosa. Median values of AA1+ cells and EG2+ cells did not differ significantly after inhalation of PAF or control (23.8 vs. 39 and 6 vs. 8/mm2, respectively, PAF vs. control, non-significant). Our findings suggest that within 24 h of inhaling a bronchoconstrictor dose of PAF, this agonist does not induce bronchial hyperresponsiveness or mucosal inflammation in atopic non-asthmatic subjects. However, because of the small number of subjects studied, these preliminary data should be interpreted with caution.
1018-2438
84-90
Lai, C.K.W.
79987e53-6fd3-4d3f-a99c-f194fea964b9
Djukanović, R.
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Wilson, J.W.
ea8d5f43-05cc-4b02-8d11-c3ba60392d11
Wilson, S.J.
21c6875d-6870-441b-ae7a-603562a646b8
Britten, K.M.
6686a738-95ba-46f8-8733-22539ad47bfc
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Lai, C.K.W.
79987e53-6fd3-4d3f-a99c-f194fea964b9
Djukanović, R.
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Wilson, J.W.
ea8d5f43-05cc-4b02-8d11-c3ba60392d11
Wilson, S.J.
21c6875d-6870-441b-ae7a-603562a646b8
Britten, K.M.
6686a738-95ba-46f8-8733-22539ad47bfc
Howarth, P.H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc

Lai, C.K.W., Djukanović, R., Wilson, J.W., Wilson, S.J., Britten, K.M., Howarth, P.H. and Holgate, S.T. (1992) Effect of inhaled platelet-activating factor on bronchial inflammation in atopic non-asthmatic subjects. International Archives of Allergy and Immunology, 99 (1), 84-90. (doi:10.1159/000236339). (PMID:1483069)

Record type: Article

Abstract

We have investigated the effect of inhaled platelet-activating factor (PAF) on bronchial mucosal inflammation in 6 atopic non-asthmatic subjects in a double-blind, placebo-controlled, randomised and crossover study. On 2 study periods at least 4 weeks apart, fiberoptic bronchoscopy was performed 24 h after inhalation of either 200 micrograms PAF or methacholine (control) to obtain endobronchial biopsies. Immunocytochemistry using antibodies for trypase (AA1) and eosinophil cationic protein (EG2) was performed to enumerate mast cells and eosinophils, respectively, in the bronchial submucosa. Median values of AA1+ cells and EG2+ cells did not differ significantly after inhalation of PAF or control (23.8 vs. 39 and 6 vs. 8/mm2, respectively, PAF vs. control, non-significant). Our findings suggest that within 24 h of inhaling a bronchoconstrictor dose of PAF, this agonist does not induce bronchial hyperresponsiveness or mucosal inflammation in atopic non-asthmatic subjects. However, because of the small number of subjects studied, these preliminary data should be interpreted with caution.

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Published date: 1992

Identifiers

Local EPrints ID: 190989
URI: https://eprints.soton.ac.uk/id/eprint/190989
ISSN: 1018-2438
PURE UUID: b7403d54-9c70-443f-84e3-4c608ab13c21
ORCID for R. Djukanović: ORCID iD orcid.org/0000-0001-6039-5612

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Date deposited: 20 Jun 2011 13:58
Last modified: 30 Jun 2018 00:36

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Contributors

Author: C.K.W. Lai
Author: R. Djukanović ORCID iD
Author: J.W. Wilson
Author: S.J. Wilson
Author: K.M. Britten
Author: P.H. Howarth
Author: S.T. Holgate

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