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A comparison of the specificity of phosphatidylcholine synthesis by human fetal lung maintained in either organ or organotypic culture

Record type: Article

Human fetal lung (14-18 weeks gestation) was maintained in either organ or organotypic culture. By 4 days in organ culture or 14 days in organotypic culture, epithelial cells within both culture systems exhibited well-developed apical microvilli and possessed numerous intracellular lamellar bodies characteristic of surfactant phospholipid stores. However, analysis of the pattern of synthesis of individual molecular species of phosphatidylcholine by [14C]choline incorporation and reversed-phase h.p.l.c. showed that this apparent maturation was not paralleled by an increased synthesis of the dipalmitoyl species in either culture system. By contrast, the fractional synthesis of dipalmitoyl phosphatidylcholine, expressed as a percentage of total [14C]choline incorporation, decreased with time in both organ and organotypic culture. Moreover, these fractions were not significantly different from those measured in parallel monolayer cultures of mixed human fetal lung cells that displayed mainly fibroblast morphology. These results suggest that the synthesis pattern of phosphatidylcholine species by lung cells in culture is determined principally by their incubation conditions and not by their state of apparent maturation.

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Citation

Caesar, Petula A., Wilson, Susan J., Normand, I. Colin S. and Postle, Anthony D. (1988) A comparison of the specificity of phosphatidylcholine synthesis by human fetal lung maintained in either organ or organotypic culture Biochemical Journal, 253, (2), pp. 451-457. (PMID:3178723).

More information

Published date: 15 July 1988

Identifiers

Local EPrints ID: 191001
URI: http://eprints.soton.ac.uk/id/eprint/191001
ISSN: 1470-8728
PURE UUID: d76233e1-cf57-4d86-ad3e-f718edeb5b95
ORCID for Anthony D. Postle: ORCID iD orcid.org/0000-0001-7361-0756

Catalogue record

Date deposited: 20 Jun 2011 12:59
Last modified: 18 Jul 2017 11:36

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Contributors

Author: Petula A. Caesar
Author: Susan J. Wilson
Author: I. Colin S. Normand

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