The University of Southampton
University of Southampton Institutional Repository

A comparison of the specificity of phosphatidylcholine synthesis by human fetal lung maintained in either organ or organotypic culture

Caesar, Petula A., Wilson, Susan J., Normand, I. Colin S. and Postle, Anthony D. (1988) A comparison of the specificity of phosphatidylcholine synthesis by human fetal lung maintained in either organ or organotypic culture Biochemical Journal, 253, (2), pp. 451-457. (PMID:3178723).

Record type: Article


Human fetal lung (14-18 weeks gestation) was maintained in either organ or organotypic culture. By 4 days in organ culture or 14 days in organotypic culture, epithelial cells within both culture systems exhibited well-developed apical microvilli and possessed numerous intracellular lamellar bodies characteristic of surfactant phospholipid stores. However, analysis of the pattern of synthesis of individual molecular species of phosphatidylcholine by [14C]choline incorporation and reversed-phase h.p.l.c. showed that this apparent maturation was not paralleled by an increased synthesis of the dipalmitoyl species in either culture system. By contrast, the fractional synthesis of dipalmitoyl phosphatidylcholine, expressed as a percentage of total [14C]choline incorporation, decreased with time in both organ and organotypic culture. Moreover, these fractions were not significantly different from those measured in parallel monolayer cultures of mixed human fetal lung cells that displayed mainly fibroblast morphology. These results suggest that the synthesis pattern of phosphatidylcholine species by lung cells in culture is determined principally by their incubation conditions and not by their state of apparent maturation.

Full text not available from this repository.

More information

Published date: 15 July 1988


Local EPrints ID: 191001
ISSN: 1470-8728
PURE UUID: d76233e1-cf57-4d86-ad3e-f718edeb5b95
ORCID for Anthony D. Postle: ORCID iD

Catalogue record

Date deposited: 20 Jun 2011 12:59
Last modified: 18 Jul 2017 11:36

Export record


Author: Petula A. Caesar
Author: Susan J. Wilson
Author: I. Colin S. Normand

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.