From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family

Wiedenmann, Jörg, Gayda, Susan, Adam, Virgile, Oswald, Franz, Nienhaus, Karin, Bourgeois, Dominique and Nienhaus, G. Ulrich (2011) From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family. Journal of Biophotonics, 4 (6), 377-390. (doi:10.1002/jbio.201000122).

Abstract

Fluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non-bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse-chase experiments and super-resolution imaging. In this review, we discuss the development of advanced variants, using a structure-function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by ?400 nm light. A variety of applications are presented that demonstrate the versatility of these marker proteins in live-cell imaging.

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Contributors

Author: Jörg Wiedenmann

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