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From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family

From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family
From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family
Fluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non-bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse-chase experiments and super-resolution imaging. In this review, we discuss the development of advanced variants, using a structure-function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by ?400 nm light. A variety of applications are presented that demonstrate the versatility of these marker proteins in live-cell imaging.
red fluorescent protein, green fluorescent protein, crystal structure, anthozoa, photoactivation
1864-063X
377-390
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Gayda, Susan
a1609aeb-7d1d-40e4-b921-65ccb2050579
Adam, Virgile
9576bdff-5f2c-4f0c-a649-e54b43949cab
Oswald, Franz
a5b02f2d-8439-411b-b5ad-999629cee58f
Nienhaus, Karin
b05bb626-b88d-48d8-b0ed-cc1624de52ca
Bourgeois, Dominique
8ba54f95-5106-47f0-bafb-83fe97c57a9d
Nienhaus, G. Ulrich
64eb2ac6-4fa9-416c-a066-f096d79307cb
Wiedenmann, Jörg
ad445af2-680f-4927-90b3-589ac9d538f7
Gayda, Susan
a1609aeb-7d1d-40e4-b921-65ccb2050579
Adam, Virgile
9576bdff-5f2c-4f0c-a649-e54b43949cab
Oswald, Franz
a5b02f2d-8439-411b-b5ad-999629cee58f
Nienhaus, Karin
b05bb626-b88d-48d8-b0ed-cc1624de52ca
Bourgeois, Dominique
8ba54f95-5106-47f0-bafb-83fe97c57a9d
Nienhaus, G. Ulrich
64eb2ac6-4fa9-416c-a066-f096d79307cb

Wiedenmann, Jörg, Gayda, Susan, Adam, Virgile, Oswald, Franz, Nienhaus, Karin, Bourgeois, Dominique and Nienhaus, G. Ulrich (2011) From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family. Journal of Biophotonics, 4 (6), 377-390. (doi:10.1002/jbio.201000122).

Record type: Article

Abstract

Fluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non-bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse-chase experiments and super-resolution imaging. In this review, we discuss the development of advanced variants, using a structure-function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by ?400 nm light. A variety of applications are presented that demonstrate the versatility of these marker proteins in live-cell imaging.

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More information

Published date: June 2011
Keywords: red fluorescent protein, green fluorescent protein, crystal structure, anthozoa, photoactivation

Identifiers

Local EPrints ID: 191501
URI: http://eprints.soton.ac.uk/id/eprint/191501
ISSN: 1864-063X
PURE UUID: d981c4ef-59f7-4d88-a405-d73c0d39fac0
ORCID for Jörg Wiedenmann: ORCID iD orcid.org/0000-0003-2128-2943

Catalogue record

Date deposited: 21 Jun 2011 14:31
Last modified: 20 Jul 2019 00:52

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