Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli
Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli
A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synthesis of very long oligonucleotides. The base sequences of the DNA strands containing this artificial linkage were copied during PCR with high fidelity and a gene containing the triazole linker was functional in Escherichia coli
11338-11343
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Sanzone, A Pia
5506750b-1f74-43af-ad9a-89ec62a6d535
Gao, Rachel
47cbe2e0-7030-4348-a6df-35673fa2a253
Tavassoli, Ali
d561cf8f-2669-46b5-b6e1-2016c85d63b2
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
27 June 2011
El-Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Sanzone, A Pia
5506750b-1f74-43af-ad9a-89ec62a6d535
Gao, Rachel
47cbe2e0-7030-4348-a6df-35673fa2a253
Tavassoli, Ali
d561cf8f-2669-46b5-b6e1-2016c85d63b2
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
El-Sagheer, Afaf H., Sanzone, A Pia, Gao, Rachel, Tavassoli, Ali and Brown, Tom
(2011)
Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli.
Proceedings of the National Academy of Sciences, 108 (28), .
(doi:10.1073/pnas.1101519108).
(PMID:21709264)
Abstract
A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synthesis of very long oligonucleotides. The base sequences of the DNA strands containing this artificial linkage were copied during PCR with high fidelity and a gene containing the triazole linker was functional in Escherichia coli
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PNAS-2011-El-Sagheer-1101519108.pdf
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Published date: 27 June 2011
Organisations:
Chemistry
Identifiers
Local EPrints ID: 191915
URI: http://eprints.soton.ac.uk/id/eprint/191915
ISSN: 0027-8424
PURE UUID: d16a8b50-02c7-4126-9a9d-ea43d20a9bfd
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Date deposited: 28 Jun 2011 07:27
Last modified: 15 Mar 2024 03:26
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Author:
Afaf H. El-Sagheer
Author:
A Pia Sanzone
Author:
Rachel Gao
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