The University of Southampton
University of Southampton Institutional Repository

Lack of evidence of a significant association between HLA-DR, DQ and DP genotypes and atopy in families with HDM allergy

Holloway, J.W., Doull, I., Begishvili, B., Beasley, R., Holgate, S.T. and Howell, W. M. (1996) Lack of evidence of a significant association between HLA-DR, DQ and DP genotypes and atopy in families with HDM allergy Clinical & Experimental Allergy, 26, (10), pp. 1142-1149. (doi:10.1111/j.1365-2222.1996.tb00500.x). (PMID:8911699).

Record type: Article

Abstract

Background: HLA class II genetic polymorphism has been variably implicated in susceptibility to specific immune responsiveness to house dust mite (HDM) allergens, and may also influence the development of atopy.

Objective: In order to assess accurately the influence of HLA alleles in the atopic immune response, we typed 22 families selected from 131 previously obtained randomly selected families (i.e. without regard to atopy or asthma), chosen on the basis of two or more members having skin prick reactivity to HDM.

Methods: Each individual was fully typed for HLA-DRB1, DQA1, DQB1 and DPB1 class II alleles using a combination of sequence-specific oligonucleotide probes (SSOP) and sequence-specific primer (SSP) polymerase chain reaction (PCR) typing and direct sequencing.

Result: Using appropriate statistical tests, no significant allelic associations were found between any DRBl, DQB1 or DPBl alleles and atopy or skin prick reactivity to Dermataphagoides pteronyssinus (Der p) or D. farinae (Der f). However, positive associations were found between DQA 1*0301 and skin prick reactivity to Der f (P= 0.009) and atopy (P= 0.027). Sib-pair analysis revealed no significant sharing of alleles between affected sib pairs for any of the phenotypes studied.

Conclusion: These results fail to confirm a previously reported association between the DRB1*04 and 07 haplotypes and atopy, and suggest that HLA class II restriction does not play a major role in the development of the IgE response to domestic house dust mite allergens in the British population.

Full text not available from this repository.

More information

Published date: October 1996
Keywords: hla, atopy, hdm allergy

Identifiers

Local EPrints ID: 192093
URI: http://eprints.soton.ac.uk/id/eprint/192093
ISSN: 0954-7894
PURE UUID: 739dd8f5-e0f8-4c55-a194-6a908f8d0af4
ORCID for J.W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464

Catalogue record

Date deposited: 29 Jun 2011 13:42
Last modified: 18 Jul 2017 11:32

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×