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A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells

A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells
A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells
There are few studies that have investigated T cell mediated lysis of adherent cells. We have developed a novel, rapid and sensitive fluorescent dye-swap assay that allows efficient detection of adherent target cell lysis. The assay allows simultaneous use of multiple differentially sensitised targets and facilitates concomitant surface or intracellular effector cell phenotypic analysis.
killing, cytotoxicity, T cell, adherent
0022-1759
153-157
Black, Antony P.
524b404d-014d-4d98-a3ad-6a73172c3b1a
Jones, Louise
68ce5626-a7bb-4856-93f3-77502a235d5e
Ardern-Jones, Michael
7ac43c24-94ab-4d19-ba69-afaa546bec90
Ogg, Graham S.
eabec6c9-1ef9-4ca6-b985-25f00a4ab358
Black, Antony P.
524b404d-014d-4d98-a3ad-6a73172c3b1a
Jones, Louise
68ce5626-a7bb-4856-93f3-77502a235d5e
Ardern-Jones, Michael
7ac43c24-94ab-4d19-ba69-afaa546bec90
Ogg, Graham S.
eabec6c9-1ef9-4ca6-b985-25f00a4ab358

Black, Antony P., Jones, Louise, Ardern-Jones, Michael and Ogg, Graham S. (2006) A novel fluorescent sensitive assay for detection of differential T cell mediated lysis of multiple adherent target cells. Journal of Immunological Methods, 316 (1-2), 153-157. (doi:10.1016/j.jim.2006.07.022). (PMID:16989854)

Record type: Article

Abstract

There are few studies that have investigated T cell mediated lysis of adherent cells. We have developed a novel, rapid and sensitive fluorescent dye-swap assay that allows efficient detection of adherent target cell lysis. The assay allows simultaneous use of multiple differentially sensitised targets and facilitates concomitant surface or intracellular effector cell phenotypic analysis.

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More information

Published date: 30 October 2006
Keywords: killing, cytotoxicity, T cell, adherent

Identifiers

Local EPrints ID: 192729
URI: http://eprints.soton.ac.uk/id/eprint/192729
ISSN: 0022-1759
PURE UUID: f5da7bbc-0a0f-43ee-94f7-341f1c2b485c

Catalogue record

Date deposited: 07 Jul 2011 12:29
Last modified: 16 Jul 2019 23:26

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